Background

Particulate matter (PM)_2.5 is a concern for public health nowadays. Although few studies have reported the skin diseases associated with PM_2.5, its effects on keratinocytes have yet to be elucidated.

Objective

The goal of this experiment was to analyze and identify the changes of gene expression in PM2.5-treated keratinocytes using RNA-sequencing (RNA-Seq) data.

Results

PM_2.5-treated keratinocytes exhibited changes in cell cycle-related genes as well as genes involved in DNA replication, endoplasmic reticulum (ER) stress, intrinsic apoptosis, and immune response. A total of 669 genes showed changes in gene expression in PM_2.5-treated keratinocytes, including 304 upregulated and 365 downregulated genes.

Conclusion

Unlike other studies investigating skin disorders associated with PM_2.5, our study found the mechanism of apoptosis suppression in keratinocytes. The findings may provide a novel insight into the management of chronic skin diseases in relation to PM_2.5.

中文翻译：

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颗粒物（PM）2.5通过内质网（ER）应激介导的凋亡抑制作用影响角质形成细胞

背景

颗粒物（PM）_2.5是当今公共卫生的关注点。尽管很少有研究报道与PM _2.5有关的皮肤疾病，但其对角质形成细胞的作用尚未阐明。

目的

该实验的目的是使用RNA测序（RNA-Seq）数据分析和鉴定PM2.5处理的角质形成细胞中基因表达的变化。

结果

PM _2.5处理的角质形成细胞在细胞周期相关基因以及与DNA复制，内质网（ER）应激，内在凋亡和免疫反应有关的基因中均表现出变化。总共669个基因在PM _2.5处理的角质形成细胞中显示出基因表达的变化，包括304个上调的基因和365个下调的基因。

结论

与其他研究与PM _2.5相关的皮肤疾病的研究不同，我们的研究发现了角质形成细胞凋亡抑制的机制。这些发现可能为与PM _2.5相关的慢性皮肤病的治疗提供新颖的见解。