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Poly-functional T helper cells in human tonsillar mononuclear cells
European Cytokine Network ( IF 2.2 ) Pub Date : 2020-03-02 , DOI: 10.1684/ecn.2015.0368
Sifei Yu 1 , Xi Luo 2 , Binyan Yang 1 , Li Xiao 1 , Xingmei Wu 3 , Huabin Li 3 , Changyou Wu 1
Affiliation  

Tonsils are important lymphoid organs in which B cells and T cells complete their maturation and identify cells that are infected by pathogens. However, the functions of T cells in human tonsils remain unclear, especially the characteristics of polyfunctional CD4+ T helper cells. In this study, we used multi-color flow cytometry to analyze the expression or co-expression of effector cytokines in CD4+ T cells from tonsillar tissues. We have demonstrated that tonsillar CD4+ T cell can express various Th effector cytokines after short-term polyclonal stimulation, and that cytokine-producing CD4+ T cells were CD45RO+ T cells. In addition, we analyzed the co-expression of two or more kinds of cytokines at the level of a single cell. The results showed that tonsillar CD4+ T cells exhibited polyfunctionality by co-expressing two to five kinds of cytokines in the same time. These data furnished a basic theory for further understanding the differentiation of polyfunctional Th cells in human tonsils and their functions in resisting invasive microorganisms.



中文翻译:


人扁桃体单核细胞中的多功能 T 辅助细胞



扁桃体是重要的淋巴器官,B 细胞和 T 细胞在其中完成成熟并识别被病原体感染的细胞。然而,人类扁桃体中T细胞的功能仍不清楚,特别是多功能CD4 + T辅助细胞的特征。在本研究中,我们使用多色流式细胞术分析来自扁桃体组织的CD4 + T细胞中效应细胞因子的表达或共表达。我们已经证明扁桃体CD4 + T细胞在短期多克隆刺激后可以表达各种Th效应细胞因子,并且产生细胞因子的CD4 + T细胞是CD45RO + T细胞。此外,我们还分析了单细胞水平上两种或多种细胞因子的共表达。结果表明,扁桃体CD4 + T细胞通过同时共表达2至5种细胞因子而表现出多功能性。这些数据为进一步了解人扁桃体中多功能Th细胞的分化及其抵抗微生物入侵的功能提供了基础理论。

更新日期:2020-03-02
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