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Evaluation of preference of brown marmorated stink bug, Halyomorpha halys (Stål) for different colour bell peppers and the role of plant protein
Arthropod-Plant Interactions ( IF 1.2 ) Pub Date : 2020-01-25 , DOI: 10.1007/s11829-020-09744-x
Melvin Mensah-Bonsu , Beatrice N. Dingha , Louis E. N. Jackai , Sarah Adjei-Fremah , Mulumebet Worku

The brown marmorated stink bug (BMSB), Halyomorpha halys, (Stål) (Hemiptera: Pentatomidae) a highly polyphagous invasive pest from Asia, has become a major agricultural pest in the United States. Laboratory experiments were conducted to determine nymph and adult BMSB preference for bell peppers of different colours (red, yellow, green, and orange). Peppers were presented to BMSB using a Dual Choice Arena Test (DCAT) with and without a standard (cowpea pod). The experiments were video recorded using the Noldus ObserverXT video system. A 4-arm olfactometer was used to determine plant volatile involvement in observed preferences. The Pierce Bicinchoninic Acid Protein assay was used to measure total protein concentration of the peppers. Results on preference measured by a Preference Index (PI, range 0–2), showed that nymphs and adults preferred peppers (PI ≥ 1.1) over cowpea pod. In a pairwise DCAT with all possible combinations, orange-coloured pepper was preferred over all the other peppers. Olfactometer test indicated that H. halys were more attracted to red and yellow peppers. Orange peppers showed the highest protein concentration whilst green peppers showed the least protein concentration.

中文翻译:

评估棕背臭臭虫(Halyomorpha halys(Stål))对不同颜色甜椒的偏爱以及植物蛋白的作用

棕色mar臭臭虫(BMSB),Halyomorpha halys(Stål)(半翅目:Pentatomidae)是一种来自亚洲的高度多食性入侵害虫,已成为美国的主要农业害虫。进行了实验室实验,以确定若虫和成年BMSB对不同颜色(红色,黄色,绿色和橙色)的甜椒的偏爱。使用双重选择竞技场测试(DCAT)将辣椒与BMSB一起使用,该标准有无标准(w豆荚)。实验是使用Noldus ObserverXT视频系统录制的。使用四臂嗅觉计确定观察到的偏好中的植物挥发性。皮尔斯比辛可宁酸蛋白质测定法用于测量辣椒的总蛋白质浓度。通过偏好指数(PI,范围为0–2)测得的偏爱结果表明,若虫和成虫比pea豆荚更喜欢辣椒(PI≥1.1)。在具有所有可能组合的成对DCAT中,橙色胡椒优于所有其他胡椒。嗅觉仪测试表明H. halys对红辣椒和黄辣椒更感兴趣。橙色胡椒显示出最高的蛋白质浓度,而绿色胡椒显示出最低的蛋白质浓度。
更新日期:2020-01-25
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