Simulated microgravity can significantly affect various cell types and multiple systems of the human body, such as cardiovascular system, skeletal muscle system, and immune system, and is known to cause anemia and loss of electrolyte and fluids. Epidermal stem cells (EpSCs) were cultured in a rotary cell culture system (RCCS) bioreactor to simulate microgravity. The metabolites of EpSCs were identified by liquid chromatography-mass spectrometry (LC-MS). Compared with normal gravity (NG) group, a total of 57 different metabolites of EpSCs were identified (P < 0.05, VIP > 1), including lipids and lipid-like molecules (51 molecules), amino acids (5 molecules), nucleosides, nucleotides, and analogues (1 molecule). According to the partial least squares discriminant analysis (PLS-DA) score plot, a VIP > 1 and P < 0.05 were obtained for the 57 different metabolites, of which 23 molecules were significantly downregulated and 34 were significantly upregulated in simulated microgravity (SMG) group. These results showed that SMG has a significant impact on different pathways, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that multiple pathways were involved, mainly the amino acid metabolism pathway, lipid metabolism pathway, membrane transport pathway, and cell growth and death pathways. Thus, the metabolic profile of EpSCs was changed under SMG. Exploring the metabolic profile of EpSCs would be helpful to further understand the growth characteristics of EpSCs under SMG, which will provide a new approach to explore the metabolomics mechanism of stress injury and repair trauma under SMG.

中文翻译：

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模拟微重力显着改变了表皮干细胞的代谢。

模拟微重力会严重影响人体的各种细胞类型和多种系统，例如心血管系统，骨骼肌系统和免疫系统，并且已知会引起贫血以及电解质和液体的流失。表皮干细胞（EpSCs）在旋转细胞培养系统（RCCS）生物反应器中培养以模拟微重力。EpSC的代谢产物通过液相色谱-质谱（LC-MS）进行鉴定。与正常重力（NG）组相比，共鉴定出57种EpSC的代谢产物（P <0.05，VIP> 1），包括脂质和类脂质分子（51个分子），氨基酸（5个分子），核苷，核苷酸和类似物（1个分子）。根据偏最小二乘判别分析（PLS-DA）得分图，VIP> 1，P <0。模拟微重力（SMG）组的57种不同代谢物获得了05，其中23个分子被显着下调，34个被显着上调。这些结果表明，SMG对不同的途径具有显着影响，《京都基因与基因组百科全书》（KEGG）途径富集分析表明涉及多个途径，主要是氨基酸代谢途径，脂质代谢途径，膜转运途径和细胞。生长和死亡途径。因此，在SMG作用下，EpSCs的代谢特征发生了改变。探索EpSCs的代谢特征将有助于进一步了解SMG下EpSCs的生长特性，这将为探索SMG下应激性损伤和修复创伤的代谢组学机制提供一种新途径。模拟微重力（SMG）组中有23个分子显着下调，有34个分子显着上调。这些结果表明，SMG对不同的途径具有显着影响，《京都基因与基因组百科全书》（KEGG）途径富集分析表明涉及多个途径，主要是氨基酸代谢途径，脂质代谢途径，膜转运途径和细胞。生长和死亡途径。因此，在SMG作用下，EpSCs的代谢特征发生了改变。探索EpSCs的代谢特征将有助于进一步了解SMG下EpSCs的生长特性，这将为探索SMG下应激性损伤和修复创伤的代谢组学机制提供一种新途径。模拟微重力（SMG）组中有23个分子显着下调，有34个分子显着上调。这些结果表明，SMG对不同的途径具有显着影响，《京都基因与基因组百科全书》（KEGG）途径富集分析表明涉及多个途径，主要是氨基酸代谢途径，脂质代谢途径，膜转运途径和细胞。生长和死亡途径。因此，在SMG作用下，EpSCs的代谢特征发生了改变。探索EpSCs的代谢特征将有助于进一步了解SMG下EpSCs的生长特性，这将为探索SMG下应激性损伤和修复创伤的代谢组学机制提供一种新途径。