Adipose tissue derived mesenchymal stem cells (ADMSCs) may be an attractive therapeutic source for acute liver injury because of their high accessibility and non-invasiveness. Here, we investigated the therapeutic potentials of porcine ADMSCs for acute liver failure (ALF). The morphology, differentiation potential, expression patterns of cell surface markers and liver-specific genes were compared between the ADMSCs derived from the pigs with or without ALF. For therapeutic studies, the expanded porcine ADMSCs from either ALF pig (ALF-ADMSCs) or healthy control pig (Nor-ADMSCs) of passage 3 were transplanted into CCl4-induced ALF mice, and the liver histology and functional tests were performed at days 1, 7, 14, and 21 after cell transplantation. ALF-ADMSCs expressed higher mRNA level of hepatic growth factor (HGF) than the Nor-ADMSCs. Both ALF-ADMSCs and Nor-ADMSCs improved liver histology, functions, and mouse survival rate. Higher level of porcine hepatocyte-specific genes was seen in the livers of ALF-ADMSCs transplanted mice as compared to the Nor-ADMSCs transplanted mice. In particular, ALF-ADMSCs transplanted mice expressed significantly higher level of albumin and cytokeratin 18 in the liver tissues as compared to the Nor-ADMSCs transplanted mice. ALF-ADMSCs might be superior to Nor-ADMSCs in the treatment of ALF as the former possesses stronger hepatic differentiation potential.

中文翻译：

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脂肪组织衍生的猪间充质干细胞可有效改善CCl4诱导的小鼠急性肝衰竭。

源自脂肪组织的间充质干细胞（ADMSC）由于其高度可及性和非侵入性，可能是急性肝损伤的有吸引力的治疗来源。在这里，我们调查了猪ADMSCs对急性肝衰竭（ALF）的治疗潜力。比较了有或没有ALF的猪的ADMSCs的形态，分化潜能，细胞表面标志物的表达模式和肝特异性基因。为了进行治疗研究，将来自第3代ALF猪（ALF-ADMSCs）或健康对照猪（Nor-ADMSCs）的扩增猪ADMSCs移植到CCl4诱导的ALF小鼠中，并在第1天进行肝组织学和功能测试，细胞移植后的7、14和21。ALF-ADMSCs表达的肝生长因子（HGF）的mRNA水平高于Nor-ADMSCs。ALF-ADMSC和Nor-ADMSC均可改善肝脏组织学，功能和小鼠存活率。与Nor-ADMSCs移植的小鼠相比，在ALF-ADMSCs移植的小鼠的肝脏中观察到更高水平的猪肝细胞特异性基因。特别地，与Nor-ADMSCs移植的小鼠相比，ALF-ADMSCs移植的小鼠在肝组织中表达的白蛋白和细胞角蛋白18的水平明显更高。在ALF的治疗中，ALF-ADMSC可能优于Nor-ADMSC，因为前者具有更强的肝分化潜能。与Nor-ADMSCs移植的小鼠相比，ALF-ADMSCs移植的小鼠在肝组织中表达的白蛋白和细胞角蛋白18明显更高。在ALF的治疗中，ALF-ADMSC可能优于Nor-ADMSC，因为前者具有更强的肝分化潜能。与Nor-ADMSCs移植的小鼠相比，ALF-ADMSCs移植的小鼠在肝组织中表达的白蛋白和细胞角蛋白18明显更高。在ALF的治疗中，ALF-ADMSC可能优于Nor-ADMSC，因为前者具有更强的肝分化潜能。