To modify a fixation method improving the intensity and clarity of the single blastomeric signal detection by fluorescence in situ hybridization (FISH) in preimplantation genetic diagnosis. 333 cycles of assisted reproduction with preimplantation genetic diagnosis FISH (PGD-FISH) performed in our hospital were analyzed and a total of 3452 single blastomeres were obtained. For the conventional fixation method, the blastomeres were kept in 0.1% sodium citrate with 0.2 mg/ml bovine serum albumin (BSA) for 2-5 min. FISH was performed and the internal relationship between embryo quality and fixed rate, signal detection rate, and signal determination rate was explored. With the modified method, 91.54% of blastomeres were fixed, while 88.30% were fixed with the conventional method. The signal detection rate was significantly increased for the modified group than for the conventional group (compared 98.53% with 94.78%, P < 0.001). Especially, the signal determination rate also showed a significant difference between the two methods (compared 90.51% with 74.17%, P < 0.001). After the development of the fixation method, the fixation efficiency and the signal determination rate were greatly improved, providing more definite diagnosis for the patient. It will hopefully allow more assisted reproduction programs to offer their patients preimplantation genetic diagnosis with FISH.

中文翻译：

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改良的单卵裂球固定技术在植入前遗传学诊断中的应用。

修改固定方法，以提高植入前遗传诊断中荧光原位杂交（FISH）检测单个卵裂球信号强度和清晰度。分析了我院进行的333个周期的辅助生殖与植入前遗传学诊断FISH（PGD-FISH），共获得3452个单卵裂球。对于常规固定方法，将卵裂球在含0.2 mg / ml牛血清白蛋白（BSA）的0.1％柠檬酸钠中保存2-5分钟。进行了FISH，探讨了胚胎质量与固定率，信号检测率和信号确定率之间的内在联系。改进方法固定了91.54％的卵裂球，而传统方法固定了88.30％。改良组的信号检出率明显高于传统组（98.53％和94.78％，P <0.001）。特别是，两种方法之间的信号确定率也显示出显着差异（分别为90.51％和74.17％，P <0.001）。随着固定方法的发展，固定效率和信号确定率大大提高，为患者提供了更加明确的诊断方法。希望它将允许更多辅助生殖计划为他们的患者提供FISH的植入前遗传学诊断。随着固定方法的发展，固定效率和信号确定率大大提高，为患者提供了更加明确的诊断方法。希望它将允许更多辅助生殖计划为他们的患者提供FISH的植入前遗传学诊断。随着固定方法的发展，固定效率和信号确定率大大提高，为患者提供了更加明确的诊断方法。希望它将允许更多辅助生殖计划为他们的患者提供FISH的植入前遗传学诊断。