MiR-1 and myostatin are markers for muscle growth and regeneration. Myostatin has a key role in the regulation of muscle mass. Myotonic dystrophy type 1(DM1) patients have a disease-specific serum miRNA profile characterized by upregulation of miR-1, miR-206, miR-133a, and miR-133b (myomiRNAs).This study aims to evaluate the possible utility of myomiRs and myostatin as biomarkers of rehabilitation efficacy in DM1, supporting clinical outcomes that are often variable and related to the patient's clinical condition.In 9 genetically proven DM1 patients, we collected biological samples before (T0) and after (T1) exercise rehabilitation training as biological measurement. We measured serum myomiRNAs by qRT-PCR and myostatin by ELISA test. The clinical outcomes measures that we utilized during a 3-6 week rehabilitation controlled aerobic exercise period were the 6-min walking test (6MWT) that increased significantly of 53.5 m (p < 0.0004) and the 10-m walk test (10MWT) that decreased of 1.38 s.We observed, after physical rehabilitation, a significant downregulation of myomiRNAs and myostatin that occurred in parallel with the improvement of clinical functional outcome measures assessed as endurance and gait speed, respectively.The modulation of biomarkers may reflect muscle regeneration and increase muscle mass after aerobic exercise. miRNAs and myostatin might be considered as circulating biomarkers of DM1 rehabilitation. The efficacy of physical rehabilitation in counteracting molecular pathways responsible for muscle atrophy and disease progression and the role of these biomarkers in DM1 and other neuromuscular diseases warrant further investigation.

中文翻译：

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MyomiRNA和myostatin作为强直性营养不良的身体康复生物标志物。

MiR-1和肌生长抑制素是肌肉生长和再生的标志。肌生长抑制素在调节肌肉质量中起关键作用。1型强直性营养不良患者（DM1）具有特定疾病的血清miRNA特征，其特征是miR-1，miR-206，miR-133a和miR-133b（myomiRNA）上调。本研究旨在评估myomiRs的可能用途。和肌生成抑制素作为DM1康复功效的生物标志物，支持经常变化且与患者的临床状况相关的临床结果。在9位经过基因证明的DM1患者中，我们在运动康复训练之前（T0）和之后（T1）收集了生物学样本测量。我们通过qRT-PCR测量了血清myomiRNA，通过ELISA测试测量了肌生长抑制素。我们在3-6周的康复控制有氧运动期间使用的临床结局指标是6分钟步行测试（6MWT）显着增加53.5 m（p <0.0004）和10分钟步行测试（10MWT）减少1.38 s。我们观察到，身体康复后，myomiRNA和myostatin显着下调，同时随着耐力和步态速度的临床功能指标的改善同时发生。生物标志物的调节可能反映肌肉再生和增加有氧运动后的肌肉质量。miRNA和肌生长抑制素可能被认为是DM1康复的循环生物标志物。