Here, QTL mapping for thousand-kernel weight carried out within a 541 × Ot1-3 population of recombinant inbred lines using high-density DArT-based map and three methods (single-marker analysis with F parametric test, marker analysis with the Kruskal–Wallis K* nonparametric test, and the recently developed analysis named genes interaction assorting by divergent selection with χ² test) revealed 28 QTL distributed over all seven rye chromosomes. The first two methods showed a high level of consistency in QTL detection. Each of 13 QTL revealed in the course of gene interaction assorting by divergent selection analysis coincided with those detected by the two other methods, confirming the reliability of the new approach to QTL mapping. Its unique feature of discriminating QTL classes might help in selecting positively acting QTL and alleles for marker-assisted selection. Also, interaction among seven QTL for thousand-kernel weight was analyzed using gene interaction assorting by the divergent selection method. Pairs of QTL showed a predominantly additive relationship, but epistatic and complementary types of two-loci interactions were also revealed.

中文翻译：

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黑麦基因组千粒重的复杂 QTL 网络。

在这里，使用基于高密度 DArT 的图谱和三种方法（使用F参数检验的单标记分析、使用 Kruskal 进行标记分析）在 541 × Ot1-3 重组自交系群体中进行了千粒重 QTL 定位。Wallis K * 非参数检验，以及最近开发的名为基因相互作用分类的分析，通过 χ ^{2 的}不同选择进行分类测试）显示 28 个 QTL 分布在所有 7 个黑麦染色体上。前两种方法在 QTL 检测中表现出高度的一致性。通过发散选择分析在基因相互作用分类过程中揭示的13个QTL中的每一个都与其他两种方法检测到的QTL一致，证实了QTL定位新方法的可靠性。其区分 QTL 类别的独特功能可能有助于选择积极作用的 QTL 和等位基因以进行标记辅助选择。此外，通过发散选择方法使用基因互作分类分析了千粒重的七个QTL之间的互作。成对的 QTL 显示出主要的加性关系，但也揭示了双基因座相互作用的上位和互补类型。