As a part of a blackleg and soft rot survey on potato plants cultivated in the agricultural region of Southern Greenland during the growing season of 2019 diseased potato plants and tubers were collected at six localities. Samples of plants and tubers with typical blackleg and soft rot symptoms were cut into parts weighing ca. 10–15 g each and incubated in potato enrichment broth (PEB) under anaerobic conditions for 4–7 days at ca. 20–22 °C (conditions during shipment of the samples from Greenland to Poland). After enrichment plant extracts were plated on crystal violet pectate (CVP) medium to isolate individual cavity-forming bacterial colonies. The inoculated plates were incubated at 22, 25 and 28 °C to maximize the chance of finding pectinolytic bacteria with different growth temperature optima. Cavity-forming isolates were collected and purified to pure cultures on tryptone soya agar (TSA). Ten cavity-forming isolates, named Green1 – Green10, representing isolates from symptomatic plants from different locations, were selected for analyses. The isolates gave a 434 bp. product in Pectobacterium spp.-specific PCR and a 439 bp. product in P. atrosepticum (Pba) -specific PCR. These isolates were Gram (−) rods, facultative anaerobic, catalase positive, oxidase and indole negative, grew in TSB + 5% NaCl, produced acid from lactose, maltose and raffinose. BlastN analyses of the ca. 1300 bp. 16S rDNA sequences of all 10 strains indicated a 99–100% similarity to the 16S rDNA of Pectobacterium atrosepticum. All 10 isolates caused soft rot of potato tuber slices after 72 h at 28 °C. Phylogenetic analysis based on the recA gene sequence grouped the isolates together with P. atrosepticum strains CFBP1526T and SCRI1043. This is the first report on isolation of P. atrosepticum from blackleg-diseased potato plants in Greenland. The presence of P. atrosepticum and its possible impact on potato cultivation in Greenland is discussed.

作为对2019年生长期在格陵兰南部农业地区种植的马铃薯植物进行黑腿和软腐调查的一部分，在六个地方收集了患病的马铃薯植物和块茎。将具有典型黑腿病和软腐病症状的植物和块茎样品切成约重约2磅的部分。每份10–15 g，并在厌氧条件下于马铃薯浓缩肉汤（PEB）中于大约70℃孵育4–7天。20–22°C（样品从格陵兰运到波兰的条件）。富集后，将植物提取物铺在果胶结晶紫果胶（CVP）培养基中，以分离单个形成腔的细菌菌落。将接种的平板在22、25和28°C下孵育，以最大程度地发现具有不同最佳生长温度的果胶分解细菌。收集形成腔的分离物，并在胰蛋白so大豆琼脂（TSA）上纯化为纯培养物。选择了十个形成腔的分离株，命名为Green1-Green10，代表来自不同位置的有症状植物的分离株。分离物产生了434bp。产品入芽孢杆菌属特异性PCR和439 bp。产物在P. atrosepticum（PBA）特异性PCR。这些分离株是革兰氏（-）杆，兼性厌氧菌，过氧化氢酶阳性，氧化酶和吲哚阴性，在TSB + 5％NaCl中生长，由乳糖，麦芽糖和棉子糖产生酸。大约BlastN分析。1300 bp。所有10个菌株的16S rDNA序列与atrosepticum杆菌的16S rDNA有99-100％的相似性。在28°C下72 h后，所有10个分离株均引起马铃薯块茎片软腐。基于recA基因序列的系统发育分析将分离株与耐腐假单胞菌菌株CFBP1526T和SCRI1043一起分组。这是关于分离到P. atrosepticum的第一份报告来自格陵兰的黑脚病马铃薯植株。讨论了atrosepticum的存在及其对格陵兰马铃薯种植的可能影响。