Quickly and easily producing uniform populations of microsphere-based 3D cell cultures using droplet-based templating methods has the potential to enable widespread use of such platforms in drug discovery or cancer research. Here, we advance the design of centrifuge-based droplet generation devices, describe the use of this platform for droplet generation with controlled cell occupancy, and demonstrate weeklong culture duration. Using simple-to-construct devices and easily implemented protocols, the initial concentration of encapsulated cells is adjustable up to hundreds of cells per microsphere. This work demonstrates the first instance of using centrifugal droplet-generating devices to produce large numbers of cell-encapsulating microspheres. Applications of this versatile methodology include the rapid formation of templated 3D cell culture populations suitable for suspension culture or large batch bioreactor studies that require uniform populations.

中文翻译：

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基于液滴的 3D 细胞培养物的离心生成。

使用基于液滴的模板方法快速、轻松地生产均匀的基于微球的 3D 细胞培养群体，有可能使此类平台在药物发现或癌症研究中得到广泛应用。在这里，我们推进了基于离心机的液滴生成设备的设计，描述了使用该平台在受控细胞占有率的情况下生成液滴，并展示了为期一周的培养持续时间。使用易于构建的设备和易于实施的协议，封装细胞的初始浓度可调节至每个微球数百个细胞。这项工作展示了使用离心液滴生成装置生产大量细胞包封微球的第一个实例。