Acoustic liquid handlers deliver small volumes (nL-µL) of multiple fluid types with accuracy and dynamic viscosity profiling. They are widely used in the pharmaceutical industry with applications extending from high-throughput screening in compound management to gene expression sequencing, genomic and epigenetic assays, and cell-based assays. The capability of the Echo to transfer small volumes of multiple types of fluids could benefit bioanalysis assays by minimization of sample volume and by simplifying dilution procedures by direct dilution. In this study, we evaluated the Labcyte Echo 525 liquid handler for its ability to deliver small volumes of sample preparations in biological matrix (plasma and serum) and to assess the feasibility of integration of the Echo with three types of bioanalytical assay platforms: microplate enzyme-linked immunosorbent assay, Gyrolab immunoassay, and liquid chromatography with tandem mass spectrometry. The results demonstrated acceptable consistency of dispensed plasma samples from multiple lots and species by the Echo. Equivalent assay performance demonstrated between the Echo and manual liquid procedures indicated great potential for the integration of the Echo with the bioanalytical assay, which allows the successful implementation of microsampling strategies in drug discovery and development.

中文翻译：

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将声学液体处理整合到生物基质样品的定量分析中。

声学液体处理器可提供小体积 (nL-µL) 的多种流体类型，并具有准确性和动态粘度曲线。它们广泛用于制药行业，应用范围从化合物管理中的高通量筛选到基因表达测序、基因组和表观遗传分析以及基于细胞的分析。Echo 传输少量多种类型流体的能力可以通过最小化样品体积和通过直接稀释简化稀释程序来有益于生物分析测定。在本研究中，我们评估了 Labcyte Echo 525 液体处理机在生物基质（血浆和血清）中输送少量样品制备物的能力，并评估了 Echo 与三种类型的生物分析分析平台集成的可行性：微孔板酶联免疫吸附测定、Gyrolab 免疫测定和液相色谱串联质谱。结果表明 Echo 从多个批次和物种中分配的血浆样品的一致性可接受。Echo 和手动液体程序之间表现出的等效测定性能表明 Echo 与生物分析测定相结合的巨大潜力，这允许在药物发现和开发中成功实施微量采样策略。