Auxin is a crucial plant growth regulator. Forward genetic screens for auxin-related mutants have led to the identification of key genes involved in auxin biosynthesis, transport, and signaling. Loss-of-function mutations in genes involved in glucosinolate biosynthesis, a metabolically related route that produces defense compounds, result in auxin overproduction. We identified an allelic series of fertile, hypomorphic Arabidopsis (Arabidopsis thaliana) mutants for the essential glucosinolate biosynthetic gene ROOTY (RTY) that exhibit a range of phenotypic defects characteristic of enhanced auxin production. Genetic characterization of these lines uncovered phenotypic suppression by cyp79b2 cyp79b3, wei2, and wei7 mutations and revealed the phenomenon of interallelic complementation in several RTY transheterozygotes. Structural modeling of RTY elucidated the relationships between structure and function in the RTY homo- and heterodimers, and unveiled the likely structural basis of interallelic complementation. This work underscores the importance of employing true null mutants in genetic complementation studies.

生长素是至关重要的植物生长调节剂。生长素相关突变体的正向遗传筛选已导致鉴定涉及生长素生物合成，转运和信号传导的关键基因。涉及芥子油苷生物合成的基因中的功能丧失突变是一种产生防御化合物的代谢相关途径，导致植物生长素的过量生产。我们确定了等位基因系列的可育，亚型拟南芥（拟南芥）突变体的基本芥子油苷生物合成基因ROOTY（RTY），表现出一系列的表型缺陷，生长素的增加。这些线路的遗传特征揭示表型抑制cyp79b2 cyp79b3，伟2和wei7突变，并揭示了几种RTY杂合子中的等位基因互补现象。RTY的结构建模阐明了RTY同二聚体和异二聚体的结构与功能之间的关系，并揭示了等位基因互补的可能的结构基础。这项工作强调了在基因互补研究中采用真正的无效突变体的重要性。