Abstract Biological control utilizing antagonistic yeasts has been actively pursued as an alternative to synthetic fungicides for the management of postharvest diseases. Abiotic stress resistance is an important attribute for antagonistic yeasts, directly associated with their biocontrol efficacy. The MADS-box transcription factor, Rlm1, has been reported to regulate the response of model yeasts to cell wall stress. Rlm1 in the antagonistic yeast, Candida oleophila, was found to play a role in resistance to salt, heat, and oxidative stress. Two Rlm1 mutants (ΔRlm1-1 and ΔRlm1-2) were generated. Compared to the wild-type (WT), C. oleophila I-182, ΔRlm1-1, and ΔRlm1-2 were more sensitive to a variety of stresses, including heat, salt, and oxidative stress. The mutants also exhibited lower biocontrol efficacy against gray mold caused by Botrytis cinerea, and slower growth in kiwifruit wounds with respect to the WT. This study provided the information to understand the relationship between the Rlm1 transcription factor, stress resistance, and biocontrol efficacy of antagonistic yeasts used for the biocontrol of postharvest diseases.

中文翻译：

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嗜油念珠菌中的 Rlm1 转录因子有助于非生物胁迫抗性和对猕猴桃采后灰霉病的生物防治功效

摘要 利用拮抗酵母的生物防治作为合成杀真菌剂的替代品用于管理收获后病害已被积极地探索。非生物胁迫抗性是拮抗酵母的一个重要属性，与其生物防治功效直接相关。据报道，MADS-box 转录因子 Rlm1 可调节模型酵母对细胞壁应激的反应。发现拮抗酵母嗜油念珠菌中的 Rlm1 在抵抗盐、热和氧化应激方面发挥作用。产生了两个 Rlm1 突变体（ΔRlm1-1 和 ΔRlm1-2）。与野生型 (WT) 相比，C. oleophila I-182、ΔRlm1-1 和 ΔRlm1-2 对各种胁迫更为敏感，包括热、盐和氧化胁迫。突变体还对灰霉病菌引起的灰霉病表现出较低的生物防治功效，并且与 WT 相比，猕猴桃伤口的生长速度较慢。本研究为了解用于收获后病害生物防治的拮抗酵母的 Rlm1 转录因子、抗逆性和生物防治功效之间的关系提供了信息。