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MicroRNA-277 regulates dopa decarboxylase to control larval-pupal and pupal-adult metamorphosis of Helicoverpa armigera.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-04-30 , DOI: 10.1016/j.ibmb.2020.103391 Zhong-Jian Shen 1 , Yan-Jun Liu 1 , Fang Zhu 2 , Li-Mei Cai 1 , Xiao-Ming Liu 1 , Zhi-Qiang Tian 1 , Jie Cheng 1 , Zhen Li 1 , Xiao-Xia Liu 1
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-04-30 , DOI: 10.1016/j.ibmb.2020.103391 Zhong-Jian Shen 1 , Yan-Jun Liu 1 , Fang Zhu 2 , Li-Mei Cai 1 , Xiao-Ming Liu 1 , Zhi-Qiang Tian 1 , Jie Cheng 1 , Zhen Li 1 , Xiao-Xia Liu 1
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Insect metamorphosis is a complex process involving many metabolic pathways, such as juvenile hormones and molting hormones, bioamines, microRNAs (miRNAs), etc. However, relatively little is known about the biogenic amines and their miRNAs to regulate cotton bollworm metamorphosis. Here we show that one miRNA, miR-277 regulates larval-pupal and pupal-adult metamorphosis of cotton bollworm by targeting the 3'UTR of Dopa decarboxylase (DDC), a synthetic catalytic enzyme of dopamine. Injection of miR-277 agomir inhibited the expression of DDC at the mRNA and protein levels, leading to defects in the pupation and emergence of H. armigera that was consistent with the phenotype obtained by injection of DDC double-stranded RNA (dsRNA). Injection of miR-277 antagomir induced the mRNA and protein expression of DDC and rescued the phenotype of pupation failure caused by DDC gene silencing. Unexpectedly, miR-277 antagomir can also cause failure of emergence of H. armigera and both agomir and antagomir of miR-277 injection could cause abnormal phenotypes in wing veins. This study reveals that elaborate regulation of miRNA and its target gene expression is prerequisite for insect development, which provides a new insight to study the developmental mechanisms of insect wing veins.
中文翻译：
MicroRNA-277调节多巴脱羧酶，以控制棉铃虫的幼虫-pu和p成人变态。
昆虫变态是一个复杂的过程，涉及许多代谢途径，例如少年激素和蜕皮激素，生物胺，微小RNA（miRNA）等。然而，对于调节棉铃虫变态的生物胺及其miRNA知之甚少。在这里，我们显示了一种miRNA，miR-277通过靶向多巴脱羧酶（DDC）的3'UTR（多巴胺的合成催化酶）来调节棉铃虫的幼虫-pu和p成人变态。注射miR-277 agomir会在mRNA和蛋白质水平上抑制DDC的表达，导致棉铃虫化脓和出现缺陷，这与注射DDC双链RNA（dsRNA）获得的表型一致。注射miR-277 antagomir可诱导DDC的mRNA和蛋白表达，并挽救因DDC基因沉默导致化脓失败的表型。出乎意料的是，miR-277 antagomir也可能导致棉铃虫的出现失败，而miR-277注射的agomir和antagomir都可能导致机翼静脉的异常表型。这项研究表明，miRNA及其靶基因表达的精细调控是昆虫发育的前提，这为研究昆虫翅膀静脉的发育机制提供了新的见识。
更新日期：2020-04-30
中文翻译：
MicroRNA-277调节多巴脱羧酶，以控制棉铃虫的幼虫-pu和p成人变态。
昆虫变态是一个复杂的过程，涉及许多代谢途径，例如少年激素和蜕皮激素，生物胺，微小RNA（miRNA）等。然而，对于调节棉铃虫变态的生物胺及其miRNA知之甚少。在这里，我们显示了一种miRNA，miR-277通过靶向多巴脱羧酶（DDC）的3'UTR（多巴胺的合成催化酶）来调节棉铃虫的幼虫-pu和p成人变态。注射miR-277 agomir会在mRNA和蛋白质水平上抑制DDC的表达，导致棉铃虫化脓和出现缺陷，这与注射DDC双链RNA（dsRNA）获得的表型一致。注射miR-277 antagomir可诱导DDC的mRNA和蛋白表达，并挽救因DDC基因沉默导致化脓失败的表型。出乎意料的是，miR-277 antagomir也可能导致棉铃虫的出现失败，而miR-277注射的agomir和antagomir都可能导致机翼静脉的异常表型。这项研究表明，miRNA及其靶基因表达的精细调控是昆虫发育的前提，这为研究昆虫翅膀静脉的发育机制提供了新的见识。
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