Small heat shock proteins (sHSPs) are a very complex protein superfamily that increase insect temperature tolerance. In order to deeply understand the function and role of sHSPs in Chilo suppressalis (Walker), this study isolated and identified two CsHSP genes lacking introns from C. suppressalis, Cshsp23.9 and Cshsp27.3. The cDNA full-length of Cshsp23.9 and Cshsp27.3 were 909 and 1 036 bp encoding 220 and 242 amino acids, respectively. Alignment with homologs and phylogenetic analysis indicated Cshsp23.9 and Cshsp27.3 were two new types of Cshsps in C. suppressalis. Real-time quantitative PCR (qPCR) revealed that Cshsp23.9 had the highest relative expression in hindgut compared with other tissues (head, epidermis, foregut, midgut, fat body, Malpighian tubules, and hemocytes), while Cshsp27.3 expressed the highest in fat body content. When exposed to thermal stress from −11 to 43°C for 2 h, two genes showed different expression patterns. Cshsp23.9 did not respond to low temperature, but could be up-regulated by high temperature and the highest expression temperature was at 36°C. Cshsp27.3 could only be induced by mild temperature, with the highest expression at 15 and 30°C. In conclusion, Cshsp23.9 and Cshsp27.3 existed in different tissues/organs of C. suppressalis, and played different important roles in C. suppressalis to resist temperature stress and regulate physiological activities.

小型热激蛋白（sHSP）是一个非常复杂的蛋白超家族，可提高昆虫的温度耐受性。为了深入了解sHSPs在抑制Chi （Walker）中的功能和作用，本研究分离并鉴定了两个来自抑制3.9的内含子的CsHSP基因Cshsp23.9和Cshsp27.3。Cshsp23.9和Cshsp27.3的cDNA全长分别为909和1036 bp，分别编码220和242个氨基酸。对准同系物和系统发育分析表明Cshsp23.9和Cshsp27.3两个新类型的Cshsps在二化螟。实时定量PCR（qPCR）揭示Cshsp23.9是具有与其他组织（头，表皮的前肠内，肠，脂肪体，马氏管，和血细胞）相比，肠最高相对表达，而Cshsp27.3表达最高体内脂肪含量高。当在-11至43°C的温度下暴露2小时时，两个基因显示出不同的表达模式。Cshsp23.9对低温没有响应，但是可以被高温上调，最高表达温度为36°C。Cshsp27.3只能由温和的温度诱导，在15和30°C时表达最高。总之，Cshsp23.9和Cshsp27.3存在于抑制suppress的不同组织/器官中，并且在抑制suppress中抵抗温度胁迫和调节生理活性中起着不同的重要作用。