We investigated the effect of S-phase kinase-associated protein 2 (SKP2) on radiosensitivity of esophageal cancer (EC) cells. Expression of SKP2, PI3K, AKT, Bcl-2 and Bax were assayed in EC. EC cells were transfected with SKP2-siRNA/IGF-1 to detect expression of SKP2, PI3K, AKT, Bcl-2 and Bax. At last, the radiosensitivity of cells in different doses of X (0, 2, 4, 6, 8 Gy) irradiation and cell apoptosis were also detected. EC cells displayed a higher positive expression rate of SKP2, elevated mRNA and protein expression of SKP2, PI3K, AKT, Bcl-2 and Bax, as well as higher extent of PI3K and AKT phosphorylation. SKP2 silencing downregulated mRNA and protein expression of PI3K, AKT and Bcl-2 but increased p27 protein expression, and inhibited the cell survival rate while promoting cell apoptosis. Taken together, silencing SKP2 can inhibit the PI3K/AKT signaling pathway, thereby increasing the radiosensitivity of EC cells.

我们研究了 S 期激酶相关蛋白 2 (SKP2) 对食管癌 (EC) 细胞放射敏感性的影响。在 EC 中测定 SKP2、PI3K、AKT、Bcl-2 和 Bax 的表达。用SKP2-siRNA/IGF-1转染EC细胞以检测SKP2、PI3K、AKT、Bcl-2和Bax的表达。最后，还检测了不同剂量X（0、2、4、6、8 Gy）照射下细胞的放射敏感性和细胞凋亡情况。EC 细胞显示出更高的 SKP2 阳性表达率，升高的 SKP2、PI3K、AKT、Bcl-2 和 Bax 的 mRNA 和蛋白质表达，以及更高程度的 PI3K 和 AKT 磷酸化。SKP2沉默下调PI3K、AKT和Bcl-2的mRNA和蛋白表达，增加p27蛋白表达，抑制细胞存活率，促进细胞凋亡。综合起来，