The first report on the use of Iodine solution for single cell release from colonial green algae Botryoccus braunii is described. The possible reaction of Iodine (I₂), Potassium iodide (KI) and the combination of both KI + I₂ solutions to release single cells embedded in the hydrocarbon-rich extracellular matrix (ECM) of B. braunii have been attempted. After these solution treatments, the algal samples were immediately viewed under a microscope to check the release of single cells and the average colonies released were counted using hemocytometer within a specific time interval. Treated colonies were initially filtered using 5 μm mesh, the remaining filtrate was passed through 0.45 μm membrane filter and the resultant organisms were subjected to various analyses. After treatment, >90% of viable cells were released by Iodine solution, without having any inhibitory effect or damage on the released cells. Compared to the above results, no cells were released by KI and < 2% cells were released and colonies were rapidly stained dark brown by KI + I₂ solution. Nile red analysis revealed that the released cells were rich in lipid bodies and the FTIR spectrum of extracted liquid hydrocarbons revealed that the presence of various forms of iodinated hydrocarbons under Iodine treatment. The experiment also proved the mechanism behind cell release by a liquid hydrocarbon, squalene, which reacted with Iodine atoms. Here, Iodine takes part to catalyst in the breakdown of double bonds to form Squalene-HI. The extracted hydrocarbons from Iodine treated shells can be utilized for pharmaceutical and cosmeceutical industries.

描述了使用碘溶液从结肠绿藻Botryoccus braunii释放单细胞的第一份报告。尝试了碘（I ₂），碘化钾（KI）和两种KI + I ₂溶液的组合可能的反应，以释放包埋在布鲁氏双歧杆菌富含烃的细胞外基质（ECM）中的单个细胞。这些溶液处理后，立即在显微镜下观察藻类样品以检查单个细胞的释放，并使用血细胞计数器在特定时间间隔内对释放的平均菌落进行计数。首先使用5μm滤网过滤处理过的菌落，将剩余的滤液通过0.45μm膜滤器，并对所得生物进行各种分析。处理后，碘溶液释放了90％以上的活细胞，而对释放的细胞没有任何抑制作用或损害。与上述结果相比，KI没有释放细胞，并且<2％的细胞没有释放，集落被KI + I ₂迅速染成深褐色解。尼罗河红分析显示释放的细胞富含脂质体，提取的液态烃的FTIR光谱表明在碘处理下存在各种形式的碘代烃。实验还证明了液态烃角鲨烯与碘原子反应释放细胞的机理。在此，碘参与双键分解形成角鲨烯-HI的催化剂。从碘处理过的贝壳中提取的碳氢化合物可用于制药和药妆工业。