The energetic compounds 1-methoxy-2,4-dinitrobenzene (DNAN), nitroguanidine (NQ), 3-nitro-1,2,4-triazole-5-one (NTO) and 1,3,5-trinitro-1,3,5-triazinane (Research Department Explosive, RDX) are widely used for explosive formulations production. Wastewater streams from industrial munition facilities are rich in nitrogen and contain considerable amounts of residual energetic compounds and metals such as zinc, nickel and copper. As such, these wastewater streams require physicochemical and/or biological treatment to meet regulatory discharge limits. The high nitrogen content of these streams makes them an attractive nutrient source for growing biomass such as microalgae. In previous studies we have identified some wastewater streams suitable for microalgal growth with minimal or no prior treatment, while others have shown moderate to high growth inhibition. The current study aims to elucidate the toxicity response of microalgae by performing growth-inhibition assays using solutions of single compounds and mixtures of energetic compounds and heavy metals. Using the freshwater green microalga Scenedesmus obliquus (S. obliquus) (ATCC® 11477) as a model organism, a microwell-plate growth-inhibition test procedure was performed to assess potential growth inhibition effects by DNAN, NQ, NTO, RDX, Zn, Ni and Cu. For the three metals tested, the order of growth rate inhibition effect on S. obliquus was Ni > Cu > Zn. The EC50s of Ni, Cu and Zn for S. obliquus were 0.08 mg/L, 0.26 mg/L and 10.13 mg/L, respectively. The effective concentrations for 50% growth inhibition (EC50) were 4.86 mg/L for DNAN and 8,700 mg/L for NTO. Conversely, RDX exhibited no toxicity below its solubility limit, and NQ had a mild toxic effect on S. obliquus with an EC20 of 303.3 mg/L.

高能化合物1-甲氧基-2,4-二硝基苯（DNAN），硝基胍（NQ），3-硝基1,2,4-三唑-5-酮（NTO）和1,3,5-三硝基-1， 3,5-三嗪烷（爆炸物研究部，RDX）被广泛用于炸药配方生产。来自工业弹药设施的废水流中富含氮，并且含有大量残留的高能化合物和金属，例如锌，镍和铜。因此，这些废水流需要进行物理化学和/或生物处理才能达到规定的排放极限。这些溪流的高氮含量使其成为用于生长诸如微藻的生物质的有吸引力的营养来源。在先前的研究中，我们已经确定了一些适合微藻生长的废水流，而这些废水几乎不需要或只需很少的处理，而另一些则显示出中到高的生长抑制作用。当前的研究旨在通过使用单一化合物的溶液以及高能化合物和重金属的混合物进行生长抑制测定来阐明微藻的毒性反应。使用淡水绿色微藻斜生栅藻（S.斜）（ ATCC®11477）作为模式生物，进行微孔板生长抑制测试程序来评估由DNAN，NQ，NTO，RDX，锌，镍和铜的潜在的生长抑制效果。对于所测试的三种金属，对斜生链球菌的生长速率抑制作用的顺序为Ni> Cu> Zn。Ni，Cu和Zn对斜纹链球菌的EC50分别为0.08 mg / L，0.26 mg / L和10.13 mg / L。对于DNAN，抑制50％生长的有效浓度（EC50）为4.86 mg / L，对于NTO为8700 mg / L。相反，RDX在其溶解度极限以下没有毒性，而NQ对斜纹链球菌有轻微的毒性作用，EC20为303.3 mg / L。