当前位置: X-MOL 学术J. Med. Mycol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Use of MALDI-TOF MS for fungal species distribution of interdigital intertrigo in seafarers, Dakar, Senegal.
Journal of Medical Mycology ( IF 2.2 ) Pub Date : 2020-04-21 , DOI: 10.1016/j.mycmed.2020.100974
K Diongue 1 , D Samb 2 , M C Seck 1 , M A Diallo 2 , M Ndiaye 1 , M D Faye 3 , A S Badiane 1 , S Ranque 4 , D Ndiaye 1
Affiliation  

To determine fungal species distribution of interdigital intertrigo among seafarers in Dakar, Senegal, a cross-sectional study was carried out from May to August 2017 among seafarers clinically diagnosed with interdigital intertrigo. A questionnaire was filled to each patient before sampling the affected folds and transporting to Aristide Le Dantec University Hospital where mycological analyses were realized. Species identification by MALDI-TOF MS was performed in Marseille, France. In total, 169 men (21–66 years) were included. Few of them (3%) had a high level of education and the duration of the mycosis exceed 10 years for 88% of patients. Direct microscopic examination (ME) was positive in 34.3%. Among samples with positive ME, 58.6% had positive culture. An overall incidence of 30.2% was found. Patients with confirmed cases aged between 28 and 66 years. Among them, those between 36–50 years were predominant (52.9%). Those with a high level of education were less representative (2%). For 52.1% of patients, the duration of the mycosis was superior to 10 years. Furthermore, 57% of cases were significantly associated with other types of tinea pedis and/or onychomycosis (P = 0.03). Culture was positive in 23.7% isolating 43 strains successfully identified at the species level by MALDI-TOF MS for 31 isolates: 20 Candida and 11 dermatophytes. The rest was identified only at the genus level belonged to Fusarium. In definitive, MALDI-TOF MS could be a useful tool for routine and fast identification of dermatophytes, yeasts and NDFF in clinical mycology laboratories.



中文翻译:

MALDI-TOF MS在塞内加尔达喀尔海员的叉指间真菌的真菌物种分布中的应用。

为了确定塞内加尔达喀尔海员之间叉指间真菌的真菌种类分布,于2017年5月至2017年8月对临床诊断为叉指间真菌的海员进行了横断面研究。在对受影响的褶皱取样并运送到阿里斯蒂德·勒·丹特克大学医院之前,已向每位患者填写了调查表,在那里进行了真菌学分析。通过MALDI-TOF MS进行的物种鉴定是在法国马赛进行的。总共包括169名男性(21-66岁)。他们中很少有人(3%)受过高等教育,88%的患者的霉菌病病程超过10年。直接显微镜检查(ME)阳性的占34.3%。在ME阳性的样本中,有58.6%的阳性培养。发现总发病率为30.2%。确诊病例的患者年龄在28至66岁之间。在这些人群中,以36至50岁为主要人群(52.9%)。受过较高教育的人代表较少(2%)。对于52.1%的患者,真菌病的持续时间优于10年。此外,57%的病例与其他类型的足癣和/或灰指甲明显相关(P  = 0.03)。通过MALDI-TOF MS在物种水平成功鉴定出43种菌株的23.7%分离株中培养阳性,其中包括31种分离株:20念珠菌和11种皮肤真菌。其余的仅在属于镰刀菌属的属水平上被鉴定。最终,MALDI-TOF MS可能是临床真菌学实验室常规和快速鉴定皮肤真菌,酵母菌和NDFF的有用工具。

更新日期:2020-04-21
down
wechat
bug