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Evaluation of MALDI-TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples.
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-04-30 , DOI: 10.1016/j.mimet.2020.105936 Thais Martins Campos Araújo 1 , Rodrigo de Castro Lisbôa Pereira 1 , Isabelle Geoffroy Ribeiro Freitag 1 , Leonardo Alves Rusak 2 , Larissa Alvarenga Batista Botelho 3 , Ernesto Hofer 1 , Cristina Barroso Hofer 4 , Deyse Christina Vallim 1
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-04-30 , DOI: 10.1016/j.mimet.2020.105936 Thais Martins Campos Araújo 1 , Rodrigo de Castro Lisbôa Pereira 1 , Isabelle Geoffroy Ribeiro Freitag 1 , Leonardo Alves Rusak 2 , Larissa Alvarenga Batista Botelho 3 , Ernesto Hofer 1 , Cristina Barroso Hofer 4 , Deyse Christina Vallim 1
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We evaluated the detection of Listeria spp. using MALDI-TOF MS directly in enrichment broths, without isolated colonies, with naturally contaminated food and stool samples. The success rate was 77%. Considering the reduced time for diagnosis and the success rate, this is a promising screening tool, but more tests are needed to determine its viability.
中文翻译：
评价MALDI-TOF MS作为检测李斯特菌的工具。直接来自食物和粪便样品的选择性富集肉汤。
我们评估了李斯特菌属的检测。将MALDI-TOF MS直接用于富集肉汤中，而无需分离菌落，并带有自然污染的食物和粪便样品。成功率为77％。考虑到减少的诊断时间和成功率，这是一种很有前途的筛选工具，但是需要更多的测试来确定其可行性。
更新日期：2020-04-30
中文翻译：
评价MALDI-TOF MS作为检测李斯特菌的工具。直接来自食物和粪便样品的选择性富集肉汤。
我们评估了李斯特菌属的检测。将MALDI-TOF MS直接用于富集肉汤中，而无需分离菌落，并带有自然污染的食物和粪便样品。成功率为77％。考虑到减少的诊断时间和成功率，这是一种很有前途的筛选工具，但是需要更多的测试来确定其可行性。
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