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Eco-friendly green synthesis of clove buds extract functionalized silver nanoparticles and evaluation of antibacterial and antidiatom activity.
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-04-21 , DOI: 10.1016/j.mimet.2020.105934
Muhammad Nazim Lakhan 1 , Rongrong Chen 1 , Altaf Hussain Shar 1 , Kishore Chand 1 , Ahmer Hussain Shah 2 , Mukhtiar Ahmed 1 , Irfan Ali 3 , Rizwan Ahmed 4 , Jingyuan Liu 1 , Kazunobu Takahashi 1 , Jun Wang 1
Affiliation  

Biological fouling has caused a lot of concern in marine industries due to the attachment of microorganisms on marine surfaces. Silver nanoparticles (AgNPs) have a great potential to inhibit and hold strong toxicity against microorganisms on artificial surfaces immersed in seawater. In this study, AgNPs are synthesized using extract of clove buds (CE) plant as a reducing and stabilizing agent by biological synthesis method. The obtained CE-AgNPs product was characterized by using different techniques. Ultraviolet-visible spectroscopy (UV-Vis) results confirmed the formation of CE-AgNPs with its surface plasmon resonance peak range. Fourier-transform infrared spectroscopy (FTIR) study showed the formation of functional groups responsible for the reduction of Ag+ into Ago. X-Ray Diffraction (XRD) results revealed face-centered cubic (fcc) silver crystals having four different diffraction peaks at 38.08, 44.21, 64.42 and 77.32 with corresponding lattice plane value recorded at (111), (200), (220) and (311), respectively. Structural characterization using scanning electron microscopy equipped with energy dispersive X-Ray Analyzer (SEM-EDX), Transmission electron microscopy (TEM) and atomic force microscopy (AFM) also confirmed the successful formation of CE-AgNPs with fcc structure. The histogram of particle size distribution through TEM image showed an average size of 9.42 nm of the synthesized product. Finally, the antibacterial and antidiatom activity of the synthesized product was analyzed. The CE-AgNPs synthesized using CE possesses good inhibitory activity against the marine bacterium community and Nitzschia closterium diatom. These results indicate that CE-AgNPs can be used as a novel material for antibacterial and antidiatoms means to inhibit the biofouling on marine surfaces.

中文翻译:

丁香芽的绿色环保合成可提取功能化的银纳米颗粒,并评估其抗菌和抗硅藻活性。

由于微生物附着在海洋表面上,生物污染在海洋工业中引起了很多关注。银纳米颗粒(AgNPs)具有很大的潜力,可以抑制并保持对浸泡在海水中的人造表面上的微生物的强烈毒性。在这项研究中,AgNPs是用丁香芽(CE)植物提取物作为还原剂和稳定剂,通过生物合成方法合成的。通过使用不同的技术表征获得的CE-AgNPs产物。紫外可见光谱(UV-Vis)结果证实了CE-AgNPs的形成及其表面等离子体共振峰范围。傅里叶变换红外光谱(FTIR)研究表明,负责将Ag +还原为Ago的官能团的形成。X射线衍射(XRD)结果显示面心立方(fcc)银晶体在38.08、44.21、64.42和77.32处具有四个不同的衍射峰,相应的晶格面值记录在(111),(200),(220)和(311)。使用配备有能量色散X射线分析仪(SEM-EDX)的扫描电子显微镜,透射电子显微镜(TEM)和原子力显微镜(AFM)进行结构表征也证实了具有fcc结构的CE-AgNP的成功形成。通过TEM图像的粒度分布直方图显示合成产物的平均尺寸为9.42nm。最后,分析了合成产物的抗菌和抗硅藻活性。使用CE合成的CE-AgNPs具有良好的抑制作用,对海洋细菌群落和Nitzschia closterium硅藻。这些结果表明,CE-AgNPs可用作抗菌和抗硅藻的新型材料,可抑制海洋表面的生物结垢。
更新日期:2020-04-21
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