Changes in the C-reactive protein (CRP) concentration, a non-specific diagnostic biomarker, in uterine lavage fluid and associations with cytological findings were examined following parturition in dairy cows. In postpartum Holstein dairy cows (n = 8), uterine lavage was performed at 3 and 6 weeks postpartum, and polymorphonuclear leukocyte (PMN) ratios to total cells (PMN:ALL) and to lymphocytes (PMN:LYM) and CRP concentrations were determined. Blood samples were collected to monitor the metabolic variables, and plasma CRP concentrations were quantified using samples collected at the same time as the uterine lavage occurred. A cytological examination was performed to determine PMN:ALL and PMN:LYM. The values for metabolic variables were within the normal range throughout the postpartum period. There was a correlation between the PMN:ALL and PMN:LYM, and both ratios were less (P < 0.05) at 6 than 3 weeks postpartum. The mean CRP concentration of the uterine lavage fluid was less at 6 (27.7 ± 9.6 ng/ml) than 3 weeks (60.8 ± 28.1 ng/ml) postpartum, whereas mean plasma CRP concentration was greater at 6 (287.4 ± 34.1 ng/ml) than 3 (254.8 ± 29.4 ng/ml) weeks postpartum. The results of the present study indicate the CRP concentration of uterine lavage fluid decreased in parallel with the frequency of PMN during uterine involution, which leads to the suggestion that uterine lavage fluid CRP concentration could be utilized as a local biomarker for evaluating uterine inflammation in cows.

中文翻译：

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量化产后奶牛子宫灌洗样本中C反应蛋白的浓度。

在分娩后，检查子宫灌洗液中C反应蛋白（CRP）浓度（一种非特异性的诊断性生物标志物）的变化以及与细胞学发现的相关性。在产后荷斯坦奶牛（n = 8）中，在产后3周和6周进行子宫灌洗，并确定多形核白细胞（PMN）与总细胞（PMN：ALL）和淋巴细胞（PMN：LYM）的比率以及CRP浓度。收集血液样本以监测代谢变量，并使用与发生子宫灌洗同时收集的样本对血浆CRP浓度进行定量。进行细胞学检查以确定PMN：ALL和PMN：LYM。整个产后，代谢变量的值均在正常范围内。PMN：ALL和PMN之间存在关联：LYM，并且两个比例在产后3周时均小于（P <0.05）。产后3天（60.8±28.1 ng / ml）的子宫灌洗液的平均CRP浓度低于6周（60.8±28.1 ng / ml），而血浆CRP的平均CRP浓度在6周（287.4±34.1 ng / ml）较大。 ）超过产后3周（254.8±29.4 ng / ml）。本研究结果表明，在子宫复旧过程中，子宫灌洗液的CRP浓度与PMN的频率平行下降，这表明建议将子宫灌洗液的CRP浓度用作评估母牛子宫炎症的局部生物标志物。 。而平均血浆CRP浓度在产后6周（287.4±34.1 ng / ml）大于3（254.8±29.4 ng / ml）。本研究结果表明，在子宫复旧过程中，子宫灌洗液的CRP浓度与PMN的频率平行下降，这表明建议将子宫灌洗液的CRP浓度用作评估母牛子宫炎症的局部生物标志物。 。而平均血浆CRP浓度在产后6周（287.4±34.1 ng / ml）大于3（254.8±29.4 ng / ml）。本研究结果表明，在子宫复旧过程中，子宫灌洗液的CRP浓度与PMN的频率平行下降，这表明建议将子宫灌洗液的CRP浓度用作评估母牛子宫炎症的局部生物标志物。 。