The objective of the study was to determine relative effects of dose (200 or 350 mg) and duration (4 or 7 days) of superstimulatory treatment on the ovarian response in prepubertal calves. Calves with similar antral follicular counts at wave emergence (n = 24) were given eight doses of either 25 or 44 mg pFSH every 12 h for 4 days or 14 doses of either 14 or 25 mg pFSH for 7 days beginning at the time of follicular wave emergence and 12.5 mg of pLH im 12 h after the last FSH treatment. On Day 4 of pFSH treatment, calves given 14 mg had fewer follicles ≥3 mm than those given 25 mg (15.1 ± 1.9 and 27.9 ± 3.3, respectively; P = 0.04). At the end of treatment (24 h post-LH), number of follicles ≥9 mm was greater in calves of groups treated with 350 than 200 mg (13.5 ± 1.8 and 8.8 ± 1.3, respectively; P = 0.02) and calves of groups treated for 7 than 4 days (13.3 ± 1.8 and 9.0 ± 1.3, respectively; P = 0.03). The number of spontaneous ovulations was greater in calves of groups treated for 7 than 4 days as was the total number of ovulations (9.7 ± 0.9 and 6.9 ± 1.0, respectively; P ≤ 0.05). In summary, a dose of 25 mg of pFSH per treatment given twice daily for 7 days resulted in a greater ovarian response than other superstimulatory treatments in prepubertal calves.

该研究的目的是确定剂量（200或350 mg）和超刺激治疗持续时间（4或7天）对青春期前犊牛卵巢反应的相对影响。从出现卵泡开始（n  = 24），每12小时给八剂剂量分别为25或44 mg pFSH的小牛出没（n = 24），共4天，或从卵泡开始起每7天给予14剂14或25 mg pFSH的小儿在最后一次FSH治疗后12小时内出现电波和12.5 mg pLH。在pFSH治疗的第4天，给予14 mg的小牛的≥3mm卵泡比给予25 mg的小（分别为15.1±1.9和27.9±3.3；P= 0.04）。在治疗结束时（LH后24小时），接受350组治疗的小牛的卵泡数量≥9mm大于200 mg（分别为13.5±1.8和8.8±1.3；P = 0.02）和各组的小牛治疗7天比4天（分别为13.3±1.8和9.0±1.3；P = 0.03）。自发排卵的数量在7超过4天治疗组的小牛了较大的作为是排卵的总数（分别为9.7±0.9和6.9±1.0; P ≤0.05）。总之，与青春期前犊牛的其他超刺激治疗相比，每天两次给予25 mg pFSH，共7天，可产生更大的卵巢反应。