Canine herpesvirus-1 (CHV-1) is recognized to be enzootic in the dog population with a widespread distribution. This pathogen leads to a lethal generalized illness in newborn puppies and is associated with reproductive disorders. CHV-1 should be considered as an important pathogen of neonatal death and infertility; so, it appears to pose a threat for breeding kennels. Although serologic data point to the circulation of CHV-1 among dogs of Iran are available, no definitive diagnosis has been conducted based on the molecular assay. So, this research was done to detect the prevalence of CHV-1 in dogs of Kerman. In this study, the presence of CHV-1 in vaginal specimens and biopsies of the uterus of dogs referring to the Veterinary Hospital of Shahid Bahonar University of Kerman was determined. Fifteen uterine samples and seven vaginal samples were included in group of the pregnant dogs. Moreover, thirteen uterine samples and twenty vaginal samples were related to the dogs displaying suspicious clinical signs such as reproductive disorders. Samples were collected and evaluated using real-time PCR. Viral DNA was detected in 21 samples from a total of 140 (15 %) collected samples which were related to 14 uterine samples (20 %) and 7 (10 %) vaginal specimens. The association of this virus with age, breed, housing, pregnancy and reproductive disorders was not significant. Five positive reproductive samples were belonged to the dogs with a history of reproductive disorders including pyometra, metritis, stillbirths, vaginitis and vaginal prolapse. This study is the first molecular detection of CHV-1 in reproductive samples of dogs in Iran. Considering the significant prevalence of this virus, it is necessary to carry out management measures in controlling and preventing this disease. Tracing CHV-1 requires further research on this virus in dogs of this region.

中文翻译：

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对伊朗东南部成年犬生殖标本中的犬疱疹病毒 1 进行的首次分子研究。


犬疱疹病毒-1 (CHV-1) 被认为是犬类中的地方性流行病，分布广泛。这种病原体会导致新生幼犬出现致命的全身性疾病，并与生殖障碍有关。 CHV-1应被视为新生儿死亡和不孕症的重要病原体；因此，它似乎对繁殖犬舍构成了威胁。尽管可获得伊朗犬类中 CHV-1 循环的血清学数据，但尚未根据分子检测进行明确诊断。因此，本研究旨在检测克尔曼犬中 CHV-1 的流行情况。在这项研究中，确定了来自克尔曼沙希德巴霍纳尔大学兽医医院的狗的阴道标本和子宫活检中是否存在 CHV-1。怀孕狗组包括十五个子宫样本和七个阴道样本。此外，十三个子宫样本和二十个阴道样本与表现出生殖障碍等可疑临床症状的狗有关。使用实时 PCR 收集和评估样品。在总共 140 个（15%）收集样本中的 21 个样本中检测到病毒 DNA，这些样本与 14 个子宫样本（20%）和 7 个（10%）阴道样本相关。这种病毒与年龄、品种、住房、怀孕和生殖障碍的关联并不显着。 5个阳性生殖样本属于有生殖疾病史的狗，包括子宫蓄脓、子宫炎、死产、阴道炎和阴道脱垂。这项研究是伊朗犬类生殖样本中首次对 CHV-1 进行分子检测。 考虑到该病毒的严重流行，有必要采取管理措施来控制和预防该疾病。追踪 CHV-1 需要在该地区的狗身上对该病毒进行进一步研究。