Abstract Polyamines (PAs), including putrescine (Put), spermidine (Spd), and spermine (Spm), play key roles in plant growth, development, and abiotic/biotic stress tolerance. Their functions appear to be intimately related to their synthesis, which occurs via arginine/ornithine decarboxylase (ADC/ODC), Spd synthase (SPDS) and Spm synthase (SPMS) etc. Previously, the transcriptional regulation of cherry under the shelter covering was carried out, and the PA synthase-related genes, particularly spermidine synthase (SPDS) gene, were differentially expressed in the fruits as exposure to the shelter covering. In the present work, the full-length cDNA sequence of this gene was cloned and named as CpSPDS (GenBank accession: MK045261). Bioinformatics analysis indicated that the cDNA sequence of CpSPDS was 1243 bp in length. Cluster analysis showed that CpSPDS had the highest homologies with those of sweet cherry (XP 021826297, 100 %) and peach (XP 020413638, 99 %). Subcellular localization detected that target gene was located in both nucleus and cytoplasm. The qPCR quantification showed that CpSPDS was differentially expressed in alabastrums, fruitlets, mature fruits and mature leaves of cherry. Interestingly, shelter covering significantly improved the fruitsetting in comparison with those from the shelter-free, gave a positive effect on the expression of CpSPDS gene. The endogenous free PAs were quantified in different tissues by high-performance liquid chromatography (HPLC). The PAs contents of the sheltered trees, i.e. Put, Spm and Spd were diversely higher in comparison to the shelter-free, especially in alabastrums, whose Spd titer of the sheltered was 3.29 folds higher than that of the unsheltered, which somehow collaborated result of mRNA quantification. Therefore, shelter covering for cherry might substantially enhance the expression of CpSPDS gene, leading to altering PAs accumulation, which at least partially contributed to the elevation in fruitsetting in rainy regions.

中文翻译：

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通过提高亚精胺合成酶 (SPDS) 基因的表达覆盖樱桃 (Prunus pseudocerasus Lindl.) 的多胺积累改变的庇护所

摘要 多胺 (PA)，包括腐胺 (Put)、亚精胺 (Spd) 和精胺 (Spm)，在植物生长、发育和非生物/生物胁迫耐受性中起关键作用。它们的功能似乎与它们的合成密切相关，通过精氨酸/鸟氨酸脱羧酶 (ADC/ODC)、Spd 合酶 (SPDS) 和 Spm 合酶 (SPMS) 等发生。并且 PA 合酶相关基因，特别是亚精胺合酶 (SPDS) 基因，在暴露于遮蔽物时在果实中差异表达。在目前的工作中，该基因的全长 cDNA 序列被克隆并命名为 CpSPDS（GenBank 登录号：MK045261）。生物信息学分析表明，CpSPDS的cDNA序列长度为1243 bp。聚类分析表明，CpSPDS 与甜樱桃（XP 021826297，100%）和桃（XP 020413638，99%）的同源性最高。亚细胞定位检测到靶基因位于细胞核和细胞质中。qPCR定量表明CpSPDS在樱桃的雪花莲、小果、成熟果实和成熟叶中差异表达。有趣的是，与无遮蔽的相比，遮蔽显着提高了坐果率，对 CpSPDS 基因的表达产生了积极影响。通过高效液相色谱 (HPLC) 对不同组织中的内源性游离 PA 进行定量。避风树的PAs含量，即Put、Spm和Spd与未避风树相比，差异很大，尤其是在雪花木中，避风树的Spd滴度为3。比未庇护的高 29 倍，这在某种程度上与 mRNA 定量的结果有关。因此，樱桃的遮蔽物可能会显着增强 CpSPDS 基因的表达，导致 PAs 积累的改变，这至少部分有助于多雨地区坐果率的提高。