Biological transmission of arthropod-borne viruses (arboviruses) to vertebrate hosts by hematophagous insects poses a global threat because such arboviruses can result in a range of serious public health infectious diseases. Sindbis virus (SINV), the prototype Alphavirus, was used to track infections in the posterior midgut (PMG) of Aedes aegypti adult mosquitoes. Females were fed viremic blood containing a virus reporter, SINV [Thosea asigna virus-green fluorescent protein (TaV-GFP)], that leaves a fluorescent signal in infected cells. We assessed whole-mount PMGs to identify primary foci, secondary target tissues, distribution, and virus persistence. Following a viremic blood meal, PMGs were dissected and analyzed at various days of post blood-feeding. We report that virus foci indicated by GFP in midgut epithelial cells resulted in a 9.8% PMG infection and a 10.8% dissemination from these infected guts. The number of virus foci ranged from 1 to 3 per individual PMG and was more prevalent in the PMG-middle > PMG-frontal > PMG-caudal regions. SINV TaV-GFP was first observed in the PMG (primary target tissue) at 3 days post blood-feeding, was sequestered in circumscribed foci, replicated in PMG peristaltic muscles (secondary target tissue) following dissemination, and GFP was observed to persist in PMGs for 30 days postinfection.

中文翻译：

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辛德毕斯病毒 (TaV-GFP) 感染在埃及伊蚊中肠的分布和持续性的共聚焦分析

通过食血昆虫将节肢动物传播的病毒（虫媒病毒）生物传播给脊椎动物宿主构成了全球威胁，因为这种虫媒病毒可导致一系列严重的公共卫生传染病。辛德毕斯病毒 (SINV)，原型甲病毒，用于跟踪中肠后部 (PMG) 的感染情况埃及伊蚊成年蚊子。给雌性喂食含有病毒报告基因 SINV 的病毒血症血液。大黄蜂病毒绿色荧光蛋白 (TaV-GFP)]，在受感染的细胞中留下荧光信号。我们评估了整体 PMG 以确定主要病灶、次要靶组织、分布和病毒持久性。在进行病毒血症血餐后，在喂血后的不同天数对 PMG 进行解剖和分析。我们报告中肠上皮细胞中 GFP 指示的病毒病灶导致 9.8% 的 PMG 感染和 10.8% 的这些感染肠道的传播。每个 PMG 的病毒病灶数量为 1 至 3 个，并且在 PMG 中部 > PMG 前部 > PMG 尾部区域更为普遍。SINV TaV-GFP 在输血后 3 天首次在 PMG（主要目标组织）中观察到，被隔离在限定的病灶中，在传播后在 PMG 蠕动肌（次要目标组织）中复制，