High-throughput microbiological experimentation using droplet microfluidics is limited due to the complexity and restricted versatility of the available detection techniques. Current detection setups are bulky, complicated, expensive, and require tedious optical alignment procedures while still mostly limited to fluorescence. In this work, we demonstrate an optofluidic detection setup for multi-parametric analyses of droplet samples by easily integrating micro-lenses and embedding optical fibers for guiding light in and out of the microfluidic chip. The optofluidic setup was validated for detection of absorbance, fluorescence, and scattered light. The developed platform was used for simultaneous detection of multiple parameters in different microbiological applications like cell density determination, growth kinetics, and antibiotic inhibition assays. Combining the high-throughput potential of droplet microfluidics with the ease, flexibility, and simplicity of optical fibers results in a powerful platform for microbiological experiments.

中文翻译：

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用于微滴中微生物样品多参数分析的光流检测装置。

由于可用检测技术的复杂性和多功能性，使用液滴微流控技术的高通量微生物实验受到限制。当前的检测装置体积庞大，复杂，昂贵，并且需要繁琐的光学对准程序，同时仍然主要限于荧光。在这项工作中，我们演示了通过轻松集成微透镜并嵌入用于引导光进出微流控芯片的光纤来对液滴样品进行多参数分析的光流检测设置。验证了光流装置可以检测吸光度，荧光和散射光。开发的平台可用于同时检测不同微生物应用中的多个参数，例如细胞密度测定，生长动力学，和抗生素抑制试验。液滴微流控技术的高通量潜力与光纤的易用性，柔韧性和简单性相结合，为微生物实验提供了强大的平台。