A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using optics such as a camera and detector. This high sensitivity is achieved by implementing 50-μm-thick reaction chambers in which a clear contrast between the RBC agglutinations and non-reacted RBCs can be obtained. The dilution and the homogenization are enhanced by injecting bubbles into the microchannel so that the test result can be obtained 5 min after the test start. With an assumption that the device will be used by medical staffs, the device is designed to require minimum operation for the users, namely, loading whole blood, starting pumps, and looking inside the reaction chambers by their eyes to observe the test result. As the device is applicable to the cross-matching test by mixing RBCs with serum instead of the reagents, it is expected that the device provides not only the quick blood-typing but also a safer and quicker blood transfusion in emergency rooms.

中文翻译：

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全自动血液分型芯片，可利用气泡进行快速稀释和检测。

开发了一种紧凑的全自动血型测试设备。该设备执行全血稀释，均质化和与试剂反应的顺序过程。芯片实验室设备可以检测红细胞（RBC）与试剂之间最弱的反应，即使不使用光学仪器（如照相机和检测器）也是如此。这种高灵敏度是通过执行来实现50- μ可以在m厚的反应室中获得RBC凝集物和未反应的RBC的清晰对比。通过向微通道中注入气泡来增强稀释和均质化，以便在测试开始后5分钟即可获得测试结果。在假设该设备将由医务人员使用的情况下，该设备被设计为要求用户进行最少的操作，即加载全血，启动泵并用眼睛注视反应室内以观察测试结果。由于该设备可通过将RBC与血清而不是试剂混合而用于交叉匹配测试，因此期望该设备不仅在急诊室中提供快速的血液分型，而且提供了更安全，更快速的输血。