Reduced blood flow, as occurring in ischemia or resulting from exposure to microgravity such as encountered in space flights, induces a decrease in the level of shear stress sensed by endothelial cells forming the inner part of blood vessels. In the present study, we use a microvasculature-on-a-chip device in order to investigate in vitro the effect of such a reduction in shear stress on shear-adapted endothelial cells. We find that, within 1 h of exposition to reduced wall shear stress, human umbilical vein endothelial cells undergo reorganization of their actin skeleton with a decrease in the number of stress fibers and actin being recruited into the cells' peripheral band, indicating a fairly fast change in the cells' phenotype due to altered flow.

中文翻译：

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剪切应力减少对内皮细胞的影响：肌动蛋白细胞骨架的微流体研究。


血流减少（如发生在缺血中或由于暴露于微重力（例如在太空飞行中遇到的情况）而导致）会导致形成血管内部的内皮细胞感受到的剪切应力水平降低。在本研究中，我们使用芯片上的微血管系统装置来体外研究剪切应力的降低对剪切适应内皮细胞的影响。我们发现，在暴露于减少的壁剪切应力的 1 小时内，人脐静脉内皮细胞的肌动蛋白骨架发生重组，应力纤维和肌动蛋白的数量减少，被招募到细胞的外周带中，这表明细胞外周带的应力纤维和肌动蛋白的数量减少，这表明人脐静脉内皮细胞的肌动蛋白骨架发生了重组。由于流动改变而导致细胞表型发生变化。