The composition and structure of the crested wheatgrass genome are still poorly understood. In the present study, using 347 F₂ individuals from the chromosome doubling of intra‐specific hybrids F₁ plants by a cross of diploid Agropyron mongplicum × Agropyron cristatum as mapping population, an effort was made to construct a high‐density genetic linkage map based on amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers and identify quantitative trait locus (QTLs) for main agronomic traits of tetraploid hybrid crested wheatgrass. The map contained 766 markers, which were ordered in 14 linkage groups, and covered a total of 2,574.3 cM with an average distance of 3.82 cM between adjacent markers. It is the genetic linkage map with the largest number of molecular markers of the crested wheatgrass and the first map for tetraploid crested wheatgrass. A total of 136 QTLs were identified for 11 traits, of which 45 were major QTLs. A total of 23 QTLs co‐localized with 14 AFLP markers and nine SSR markers, respectively, and showed a great potential for marker‐assisted selection in improving these traits. A total of 30 QTLs showed pleiotropism, and the consensus QTLs were further integrated into seven pleiotropic unique QTLs. The significant co‐localization of QTLs was consistent with the significant phenotypic correlations among these traits. The results lay a foundation for map‐based cloning, functional analysis and designing molecular breeding of favorable genes in crested wheatgrass of further study.

中文翻译：

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高倍遗传连锁图谱的构建及四倍体杂种冠毛草主要农艺性状的QTL鉴定

凤头小麦草基因组的组成和结构仍然知之甚少。在本研究中，使用来自二倍体Agropyron mongplicum  ×  Agropyron cristatum杂交的种内杂种F ₁植物染色体倍增的347个F ₂个体作为作图种群，人们努力构建基于扩增片段长度多态性（AFLP）和简单序列重复（SSR）标记的高密度遗传连锁图谱，并确定四倍体杂种c的主要农艺性状的数量性状位点（QTL）。小麦草。该图包含766个标记，按14个连锁组的顺序排列，覆盖总计2,574.3 cM，相邻标记之间的平均距离为3.82 cM。这是有顶冠小麦草分子标记数量最多的遗传连锁图谱，也是四倍体冠顶小麦草的第一个图谱。共鉴定出11个性状的136个QTL，其中45个为主要QTL。共有23个QTL与14个AFLP标记和9个SSR标记共定位，并显示出在改进这些性状上进行标记辅助选择的巨大潜力。共有30个QTL表现出多效性，并且共有QTL被进一步整合为7个多效性独特QTL。QTL的显着共定位与这些性状之间的显着表型相关。该结果为进一步研究冠毛小麦草的基于图的克隆，功能分析和设计有利基因的分子育种奠定了基础。