Fecal progesterone and estrogen metabolite monitoring for cyclicity and pregnancy in southern tamandua (Tamandua tetradactyla ),Zoo Biology

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Fecal progesterone and estrogen metabolite monitoring for cyclicity and pregnancy in southern tamandua (Tamandua tetradactyla )
Zoo Biology ( IF 1.2 ) Pub Date : 2020-04-09 , DOI: 10.1002/zoo.21542
Kim Daly-Crews ₁ , R Harrison Edell ₂ , Lara C Metrione ₁

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Reproductive management of the southern tamandua (Tamandua tetradactyla) should include timed introductions for breeding to minimize aggression and pregnancy monitoring. Since serial blood sampling could cause unnecessary stress, and urinary progesterone metabolites are found in very low concentrations, this study sought to validate progesterone and estradiol enzyme immunoassays for measuring fecal progesterone metabolite (FPM) and fecal estrogen metabolite (FEM) concentrations in two females. Peaks in FEM concentrations coincided with breeding and conception, were 5-6 times higher than baseline concentrations, and were followed by clear luteal phases distinguished by FPM concentrations 5-6 times higher than baseline concentrations. FPM concentrations during the first 30-53 days of gestation overlapped with luteal phase concentrations, thereafter increasing to 8-25 times higher than baseline concentrations. FEM concentrations during the first 41-44 days of gestation remained near basal values for one female, whereas concentrations were 1.8 times higher than baseline for the second. FEM concentrations became elevated for the former by 44 days of gestation and increased further for the latter after 53 days, ultimately averaging four times higher than baseline for both females. The biphasic increase in FPM and FEM concentrations, follicular and luteal phase durations (follicular: 7 ± 1 days, luteal: 25 ± 1 days), total cycle length (41 ± 1 days), and gestation (161-165 days) documented in this study were consistent with previous reports from serum and urine analyses. Monitoring FPM and FEM is a reliable noninvasive method for tracking reproductive cycles and pregnancy in southern tamandua that overcomes the challenges associated with serum or urinary hormone analysis.

中文翻译：

在南塔曼杜瓦（Tamandua tetradactyla）中监测周期性和妊娠的粪便孕酮和雌激素代谢物

南部 tamandua (Tamandua tetradactyla) 的生殖管理应包括定时引入繁殖，以尽量减少侵略和妊娠监测。由于连续采血可能会导致不必要的压力，而且尿中孕酮代谢物的浓度非常低，因此本研究试图验证孕酮和雌二醇酶免疫测定法，用于测量两名女性的粪便孕酮代谢物 (FPM) 和粪便雌激素代谢物 (FEM) 浓度。FEM 浓度的峰值与繁殖和受孕一致，比基线浓度高 5-6 倍，然后是清晰的黄体期，FPM 浓度比基线浓度高 5-6 倍。妊娠前 30-53 天的 FPM 浓度与黄体期浓度重叠，此后增加到比基线浓度高 8-25 倍。一名女性在妊娠前 41-44 天的 FEM 浓度保持接近基础值，而第二名的浓度比基线高 1.8 倍。前者的 FEM 浓度在妊娠 44 天时升高，后者在 53 天后进一步增加，最终平均比基线高四倍。FPM 和 FEM 浓度、卵泡期和黄体期持续时间（卵泡期：7 ± 1 天，黄体期：25 ± 1 天）、总周期长度（41 ± 1 天）和妊娠期（161-165 天）的双相增加记录在该研究与之前的血清和尿液分析报告一致。

更新日期：2020-04-09

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