Generation and characterization of inner photoreceptor-specific enhancer-trap lines using a novel piggyBac-Gal4 element in Drosophila.,Archives of Insect Biochemistry and Physiology

当前位置： X-MOL 学术 › Arch. Insect Biochem. Physiol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Generation and characterization of inner photoreceptor-specific enhancer-trap lines using a novel piggyBac-Gal4 element in Drosophila.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-04-13 , DOI: 10.1002/arch.21675
Ece Terzioğlu Kara ₁ , Ferdi Rıdvan Kiral _{1,

2} , Arzu Öztürk Çolak ₁ , Arzu Çelik _{1,

3}

Affiliation

The Drosophila inner photoreceptors R7 and R8 are responsible for color vision and their differentiation starts at the third instar larval stage. Only a handful of genes with R7 or R8‐cell‐specific expression are known. We performed an enhancer‐trap screen using a novel piggyBac transposable element, pBGay, carrying a Gal4 sequence under the control of the P promoter to identify novel genes expressed specifically in R7 or R8 cells. From this screen, three lines were analyzed in detail: piggyBac^AC109 and piggyBac^AC783 are expressed in R8 cells and piggyBac^AC887 is expressed in R7 cells at the third instar larval stage and pupal stages. Molecular analysis showed that the piggyBac elements were inserted into the first intron of CG14160 and CG7985 genes and the second intron of unzipped. We show the expression pattern in the developing eye imaginal disc, pupal retina as well as the adult retina. The photoreceptor‐specific expression of these genes is reported for the first time and we propose that these lines are useful tools for studying the development of the visual system.

中文翻译：

使用果蝇中的新型piggyBac-Gal4元件生成内部感光受体特异性增强子陷阱系并对其进行表征。

在果蝇内感光R7和R8负责色觉和其分化为三龄幼虫期开始。只有少数具有R7或R8细胞特异性表达的基因是已知的。我们使用新型的piggyBac转座因子pBGay进行了增强子捕获筛选，该载体在P启动子的控制下携带Gal4序列，以鉴定在R7或R8细胞中特异性表达的新型基因。从该屏幕上，详细分析了三行：在第三龄幼虫期和^p期，piggyBac ^AC109和piggyBac ^AC783在R8细胞中表达，piggyBac ^AC887在R7细胞中表达。分子分析表明将piggyBac元件插入CG14160和CG7985基因的第一个内含子和解压缩的第二个内含子。我们在发育中的眼球间盘，p视网膜以及成年视网膜中显示表达模式。首次报道了这些基因的光感受器特异性表达，我们认为这些细胞系是研究视觉系统发育的有用工具。

更新日期：2020-04-13

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11