We taxonomically examined an algal strain (Ki‐4) isolated from a dry asphalt surface in mid‐summer of 2003. The vegetative cells were solitary, covered by a cell wall ornamented with meridional ribs, and contained hollow spherical chloroplasts and a pyrenoid. The cells exhibited a high tolerance to unfavorable photo‐oxidative stress conditions, and in culture developed a reddish coloration due to the accumulation of a water‐soluble astaxanthin‐binding protein, which was identified as a novel protein in organisms. 18S rDNA and ITS2 sequence analyses revealed that Ki‐4 belongs to Coelastrella sensu lato (Scenedesmaceae), but was taxonomically distinct from other members of the genus. On the basis of its phylogenetic position and morphological features, including the structures of cell wall ribs, we identify Ki‐4 as a new algal species in the genus Coelastrella, for which we propose the name Coelastrella astaxanthina sp. nov.

中文翻译：

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Coelastrella astaxanthina sp. 十一月 (Sphaeropleales, Chlorophyceae)，一种在日本盛夏从沥青表面分离的新型微藻

我们对 2003 年仲夏从干燥沥青表面分离的藻类菌株 (Ki-4) 进行了分类学检查。 营养细胞是孤立的，被装饰有经向肋的细胞壁覆盖，并含有空心球形叶绿体和核糖体。这些细胞对不利的光氧化应激条件表现出高度耐受性，并且在培养中由于水溶性虾青素结合蛋白的积累而呈现微红色，该蛋白被鉴定为生物体中的一种新型蛋白质。18S rDNA 和 ITS2 序列分析显示 Ki-4 属于 Coelastrella sensu lato（Scenedesmaceae），但在分类学上与该属的其他成员不同。根据其系统发育位置和形态特征，包括细胞壁肋的结构，我们将 Ki-4 确定为 Coelastrella 属中的一个新藻类，为此我们建议将其命名为 Coelastrella astaxanthina sp。十一月