This study was conducted to identify stable resistance to net form of net blotch (NFNB) in spring barley in Moroccan environments. Seedling resistance to NFNB was evaluated by inoculating 336 barley genotypes with two NFNB isolates LDNH04Ptt‐19 and TD‐10 in the greenhouse. These genotypes were evaluated for adult plant resistance to NFNB under seven environments in Morocco in 2015 and 2016. The disease severity was estimated at GS 77–87 on barley leaves using a double‐digit scale. To investigate stability of resistance, 149 barley genotypes were subjected to AMMI analysis. At the seedling stage, differential responses of barley genotypes to different NFNB isolates were identified, whereas genotypes had variable stability to NFNB resistance at the adult stages. Five genotypes, AM‐68, AM‐95, AM‐250, AM‐267 and AM‐322, were resistant to both NFNB isolates at the seedling stage. There were significant (p < .001) effects of genotype (G) and G × E interaction on NFNB severity for barley genotypes at the adult stage. The principal components, IPCA1 and IPCA2, accounted for 48.4% and 18.7% variation for NFNB severity, respectively. The AMMI stability values (ASVs) ranged from 0.01 to 15.5, and fifty‐nine barley genotypes had stable responses (ASV ≤ 0.05) across all seven environments. Specifically, two stable genotypes, AM‐187 and AM‐244, had lower mean NFNB severities across all environments, suggesting a quantitative resistance in these genotypes. Divergent environmental responses of NFNB severity were measured in Sidi El Ayedi 2015 and Sidi Allal Tazi 2016, suggesting that these environments may be suitable to capture resistance to diverse pathotypes. These stable genotypes are valuable resources for introgression of both qualitative resistance and quantitative resistance to NFNB in future.

中文翻译：

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摩洛哥春大麦幼苗和成年期对网斑病（NFNB）的抗性以及成年期对NFNB的抗性稳定性

本研究旨在确定摩洛哥环境中春大麦对网状网斑病 (NFNB) 的稳定抗性。通过在温室中用两种 NFNB 分离株 LDNH04Ptt-19 和 TD-10 接种 336 个大麦基因型来评估幼苗对 NFNB 的抗性。2015 年和 2016 年在摩洛哥的 7 个环境下评估了这些基因型对 NFNB 的成株抗性。使用两位数的比例估计大麦叶片的疾病严重程度为 GS 77-87。为了研究抗性的稳定性，对 149 个大麦基因型进行了 AMMI 分析。在幼苗阶段，鉴定了大麦基因型对不同 NFNB 分离株的不同反应，而在成年阶段，基因型对 NFNB 抗性具有不同的稳定性。五种基因型，AM-68、AM-95、AM-250、AM-267 和 AM-322，在幼苗阶段对两种 NFNB 分离株都具有抗性。对于成年阶段的大麦基因型，基因型 (G) 和 G × E 相互作用对 NFNB 严重程度有显着影响 (p < .001)。主要成分 IPCA1 和 IPCA2 分别占 NFNB 严重性变异的 48.4% 和 18.7%。AMMI 稳定性值 (ASV) 的范围从 0.01 到 15.5，并且 59 个大麦基因型在所有七个环境中具有稳定的响应 (ASV ≤ 0.05)。具体来说，两种稳定的基因型 AM-187 和 AM-244 在所有环境中的平均 NFNB 严重程度较低，表明这些基因型存在数量上的抗性。在 Sidi El Ayedi 2015 和 Sidi Allal Tazi 2016 中测量了 NFNB 严重程度的不同环境反应，表明这些环境可能适合捕获对不同病理类型的抗性。