In this study, the antifungal effects of six different isothiocyanate (ITCs) compounds (methyl, allyl, butyl, ethyl, benzyl and 2‐phenylethyl ITCs) were investigated to be use against the citrus sour rot disease caused by Geotrichum citri‐aurantii in vitro and semi‐commercial (in vivo) conditions. Antifungal activities of the vapour phases of different ITC compounds were examined on the arthroconidia germination and mycelial growth of G. citri‐aurantii. Mycelial growth of G. citri‐aurantii was inhibited in a concentration‐dependent way. The minimum inhibitory concentrations of benzyl, methyl, allyl and ethyl ITCs on mycelial growth were 0.06, 0.08, 0.10 and 0.10 µl/L, respectively. Arthroconidia germination of G. citri‐aurantii was completely inhibited by benzyl, methyl, allyl and ethyl ITCs at concentrations of 0.05, 0.07, 0.07 and 0.07 µl/L, respectively. Light microscopy observations revealed that the ITC compounds, at completely inhibiting concentrations, caused considerable morphological changes in the fungal hyphae. Under in vivo conditions, the average rotting area caused by G. citri‐arantii was inhibited 100% by ethyl, methyl and allyl ITC compounds at concentrations of 8.0, 12.0 and 12.0 µl/L, respectively. Results suggest that ITC’s may be useful and effective natural antifungal compounds to control the citrus sour rot disease agent.

中文翻译：

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硫代葡萄糖苷衍生的异硫氰酸酯作为潜在的天然抗真菌化合物对柑橘酸腐病病原体 Geotrichum citri-aurantii 的评估

在这项研究中，研究了六种不同的异硫氰酸酯 (ITCs) 化合物（甲基、烯丙基、丁基、乙基、苄基和 2-苯乙基 ITCs）在体外对抗由黄地霉引起的柑橘酸腐病的抗真菌作用和半商业（体内）条件。研究了不同 ITC 化合物气相对 G. citri-aurantii 的节孢子萌发和菌丝体生长的抗真菌活性。G. citri-aurantii 的菌丝生长受到浓度依赖性的抑制。苄基、甲基、烯丙基和乙基 ITCs 对菌丝生长的最小抑制浓度分别为 0.06、0.08、0.10 和 0.10 µl/L。在浓度为 0.05、0.07、0.07 和 0.07 µl/L 时，苄基、甲基、烯丙基和乙基 ITC 完全抑制了 G. citri-aurantii 的节孢子菌萌发，分别。光学显微镜观察表明，ITC 化合物在完全抑制浓度下会导致真菌菌丝发生相当大的形态变化。在体内条件下，浓度分别为 8.0、12.0 和 12.0 µl/L 的乙基、甲基和烯丙基 ITC 化合物可 100% 抑制由 G. citri-arantii 引起的平均腐烂面积。结果表明，ITC's 可能是一种有用且有效的天然抗真菌化合物，可用于控制柑橘酸腐病病原体。分别为 0 和 12.0 µl/L。结果表明，ITC's 可能是一种有用且有效的天然抗真菌化合物，可用于控制柑橘酸腐病病原体。分别为 0 和 12.0 µl/L。结果表明，ITC's 可能是一种有用且有效的天然抗真菌化合物，可用于控制柑橘酸腐病病原体。