Acilius kishii Nakane, 1963 (Coleoptera: Dytiscidae) is an endangered diving beetle species distributed in only one location, Lake Yashaga‐Ike, Honshu Island, Japan. Acilius japonicus, which is related to A. kishii, is distributed widely in northern Honshu Island and Hokkaido Island in Japan. In this study, we identified 14 microsatellite loci for A. kishii and A. japonicus, including both polymorphic and monomorphic loci, using the next‐generation sequencing method. We observed that 5 and 10 loci showed polymorphisms in 31 and 32 individuals of A. kishii and A. japonicus, respectively. The observed and expected heterozygosities were 0.00–1.00 and 0.00–0.74, respectively. These microsatellite loci could be useful for future conservation genetic studies, including monitoring of genetic diversity and extinction risk of A. kishii.

中文翻译：

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为极有限分布的稀有潜水甲虫 Acilius kishii 和广泛分布的 Acilius japonicus（鞘翅目：Dytiscidae）开发微卫星标记

Acilius kishii Nakane，1963（鞘翅目：Dytiscidae）是一种濒临灭绝的潜水甲虫物种，仅分布在日本本州岛八世加池的一个地方。Acilius japonicus与A. kishii有亲缘关系，广泛分布于日本本州岛北部和北海道岛。在这项研究中，我们使用下一代测序方法确定了 A. kishii 和 A. japonicus 的 14 个微卫星位点，包括多态性和单态性位点。我们观察到 5 个和 10 个基因座分别在 31 和 32 个 A. kishii 和 A. japonicus 个体中显示出多态性。观察到的和预期的杂合度分别为 0.00-1.00 和 0.00-0.74。这些微卫星位点可用于未来的保护遗传研究，包括监测 A. kishii 的遗传多样性和灭绝风险。