Evaluation and monitoring of stocking effectiveness is essential to make informed fisheries management decisions. Chemical marking, such as oxytetracycline (OTC), is often used for differentiating stocked from wild fish but has inherent error in mark longevity and otolith evaluation. Genetic analysis of recaptures provides a viable alternative. In this study, fingerlings (~50 mm TL) of F₁ intergrade Largemouth Bass Micropterus salmoides (Florida Largemouth Bass M. salmoides floridanus × Northern Largemouth Bass M. salmoides salmoides) were marked with OTC via immersion and stocked into the Chickahominy River, Virginia, from 2005 to 2007 and more recently from 2015 to 2017, totaling 595,000 fish. We collected Largemouth Bass in 2015 and 2017 during long‐term monitoring on the tidal portion of the Chickahominy River in Virginia. Fish were retained for aging, OTC evaluation, and genetic analysis. Although precision (i.e., agreement) was high (95%) when comparing the two methods, by establishing a baseline number of known recaptures (N = 19 samples verified by genetics), we determined that detection probability (accuracy) was 63%. Differentiating stocked from wild fish with genetics provides advantages over OTC immersion for stocking evaluations, including potential cost and labor savings. Future studies should routinely incorporate an evaluation of tagging detection rate to accurately adjust percent contribution estimates.

中文翻译：

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土霉素和F1基因与野生大嘴鲈种群分化的实证评估

评估和监测放养效果对于做出明智的渔业管理决定至关重要。化学标记，例如土霉素（OTC），通常用于区分野生鱼类和家畜，但在标记寿命和耳石评估方面存在固有误差。捕获物的遗传分析提供了可行的选择。在这项研究中，F ₁过渡级大嘴鲈鱼小鳞p的鱼种（〜50 mm TL）（佛罗里达大嘴鲈鱼M. salmoides floridanus  ×北大嘴鲈鱼M. salmoides salmoides））在2005年至2007年以及最近的2015年至2017年期间，通过浸泡在OTC上进行了标记并放养到弗吉尼亚州的奇卡米米尼河中，总计595,000条鱼。我们在2015年和2017年对弗吉尼亚州奇克阿霍米尼河的潮汐部分进行了长期监测，收集了大嘴鲈。保留鱼以进行老化，OTC评估和遗传分析。尽管比较这两种方法时的准确度（即一致性）很高（95％），但通过确定已知捕获的基线数量（N=通过遗传学验证的19个样本），我们确定检测概率（准确性）为63％。通过遗传学将野生鱼类与家畜区分开来，比OTC浸泡更具优势，可进行种群评估，包括潜在成本和劳动力节省。未来的研究应例行地对标记检测率进行评估，以准确调整贡献百分比估算。