In the present study, juvenile (live body weight, 54.3 ± 8.2 g), preadult (live body weight, 822.5 ± 33.9 g), and adult (live body weight, 1,562.8 ± 41.8 g) pacu, Piaractus mesopotamicus, were used to estimate their dietary essential amino acid (EAA) requirements using the whole‐body amino acid (AA) pattern. The results showed that whole‐body moisture, crude protein, total lipid, and ash contents expressed on a wet weight basis (%) were significantly different among the studied growth phases. No significant differences were observed in the dietary EAA requirements estimated for the three growth phases of pacu. These dietary EAA requirements were found to be different than those previously estimated for the same fish through its muscle AA pattern. Based on whole‐body EAA to total EAA ratios {A/E ratios; [(each EAA/total EAA) × 1,000]}, EAA requirements were estimated to be histidine (0.42%), arginine (1.36%), threonine (0.82%), valine (0.90%), methionine (0.45%), isoleucine (0.83%), leucine (1.29%), phenylalanine (0.74%), lysine (1.64%), and tryptophan (0.14%) for pacu. These estimated requirements may serve as a reference line in the formulation of practical and experimental diets until dose–response‐based optimum EAA requirements are available for pacu.

中文翻译：

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幼年，成年前和成年幼体，Piaractus mesopotamicus的全身氨基酸模式，并估计其饮食中必需氨基酸的含量

在本研究中，幼年（活体重54.3±8.2克），成年前（活体重822.5±33.9克）和成人（活体重1,562.8±41.8克）pacu，美索不达米亚（Piaractus mesopotamicus），通过全身氨基酸（AA）模式来估算其饮食必需氨基酸（EAA）需求。结果表明，在研究的生长期之间，以湿重（％）表示的全身水分，粗蛋白，总脂质和灰分含量显着不同。在估计的pacu的三个生长阶段的饮食EAA需求中没有观察到显着差异。发现这些饮食中的EAA要求与先前通过其肌肉AA模式对同一条鱼所估计的不同。基于全身EAA与总EAA比率{A / E比率；[（每个EAA /总EAA×1000）}}，估计EAA需求量为组氨酸（0.42％），精氨酸（1.36％），苏氨酸（0.82％），缬氨酸（0.90％），蛋氨酸（0.45％），异亮氨酸（0.83％），亮氨酸（1.29％），苯丙氨酸（0.74％），赖氨酸（1。64％），色氨酸（0.14％）用于pacu。这些估计的需求量可以作为制定实际和实验饮食的参考线，直到可用于pacu的基于剂量反应的最佳EAA需求量为止。