To investigate the effects of supplemental nutrient additives in broad bean‐based diets on growth, flesh quality, and collagen gene expression of grass carp, five diets were prepared, including complete formula diet (control), soaked broad bean (SBB), and three broad bean‐based diets containing 80% broad bean without (BBD1) or with the supplementation of methionine (BBD2), and methionine+vitamins+minerals (BBD3), and were fed to grass carp (171.9 ± 1.1 g), Ctenopharyngodon idellus, for 84 days. The results showed that broad bean‐based diets significantly increased weight gain (WG) and reduced the feed conversion ratio (FCR) of grass carp when compared to the SBB (p < .05). The BBD3 group reached levels similar to the WG and FCR of the control group (p > .05). Grass carp fed the BBD3 diet had lower steaming loss of flesh, higher muscle fiber density, and higher collagen content in muscle and skin than the control (p < .05). The relative expressions of COL1A1 and COL1A2 mRNA in muscle and skin were significantly higher in the BBD3 and SBB groups than in other groups (p < .05). In conclusion, the combination of methionine, vitamins, and minerals in broad bean‐based diets promoted the growth of “crisped” grass carp and improved flesh quality and collagen gene expression when compared to the control formula diet.

中文翻译：

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在蚕豆饮食中补充营养添加剂可改善“脆皮”草鱼的生长。

为了研究蚕豆日粮中补充营养添加剂对草鱼生长，肉质和胶原基因表达的影响，准备了五种日粮，包括全配方日粮（对照），浸泡蚕豆（SBB）和三种以蚕豆为基础的日粮，其中含有80％的蚕豆，不含（BBD1）或不添加蛋氨酸（BBD2），以及蛋氨酸+维生素+矿物质（BBD3），并喂给草鱼（171.9±1.1 g），C鱼（Ctenopharyngodon idellus），持续84天。结果表明，与SBB相比，以蚕豆为基础的日粮显着提高了草鱼的增重（WG）和降低了饲料转化率（FCR）（p  <.05）。BBD3组达到了与对照组的WG和FCR相似的水平（p > .05）。饲喂BBD3日粮的草鱼与对照组相比，肉的蒸煮损失少，肌肉纤维密度高，胶原蛋白含量高（p  <.05）。在BBD3和SBB组中，肌肉和皮肤中COL1A1和COL1A2 mRNA的相对表达明显高于其他组（p  <.05）。总之，与对照配方食品相比，蚕豆粗粮中蛋氨酸，维生素和矿物质的结合促进了“脆皮”草鱼的生长，并改善了肉质和胶原基因表达。