Two shrimp genetic lines (LGR: faster growth and LWR: higher white spot syndrome virus resistance) were evaluated with six dietary treatments containing different sources (animal and plant), and three protein levels (low, medium and high). A five‐week bioassay was conducted using Litopenaeus vannamei with an average initial weight of 2.08 ± 0.06 g to determine the influence of the genetic line, protein source, and protein level on the weight gained per week, survival, and expression of immune‐related genes. The overall mean of weight gain was 1.02 g/week for LGR and 0.71 g/week for LWR. Survival was 71.7% for LGR and 78.9% for LWR. The genetic line influenced weekly weight gain and survival while a significant interaction between genetic line, protein source, and protein level, influenced the expression of immune‐related genes of shrimp. The data suggested that a diet containing plant‐based protein (30–35%) at medium level was sufficient to obtain adequate values of weekly weight gain and survival without affecting the immune response. However, LGR showed higher weight gain, and LWR increased survival and gene expression levels related to the immune response. These results could be of interest on feed formulation for aquatic organisms and feeding strategies of farmed shrimp.

中文翻译：

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遗传系，蛋白质来源和蛋白质水平对凡纳滨对虾生长，存活和免疫相关基因表达的影响

用六种不同来源（动物和植物）和三种蛋白质水平（低，中和高）的饮食疗法评估了两个虾类遗传系（LGR：生长更快，LWR：更高的白斑综合症病毒抗性）。使用南美白对虾进行了为期五周的生物测定初始平均体重为2.08±0.06 g，以确定遗传系，蛋白质来源和蛋白质水平对每周增加的体重，存活率和免疫相关基因表达的影响。LGR的总增重平均值为1.02 g /周，LWR为0.71 g /周。LGR的生存率为71.7％，LWR的生存率为78.9％。遗传系影响每周的体重增加和存活，而遗传系，蛋白质来源和蛋白质水平之间的显着相互作用影响了虾免疫相关基因的表达。数据表明，在中等水平的饮食中含有植物性蛋白质（30–35％）足以获得足够的每周体重增加和生存值，而不会影响免疫反应。但是，LGR的体重增加了，LWR和LWR增加了与免疫反应有关的存活率和基因表达水平。这些结果可能对水生生物的饲料配方和养殖对虾的饲喂策略有意义。