This study aimed to assess the effects of replacing fish meal with cottonseed meal protein hydrolysate (CPH) on the growth, intestinal function, growth hormone/insulin‐like growth factor I (GH/IGF‐I) axis, and inflammation of blunt snout bream, Megalobrama amblycephala. A total of 300 fish (38.66 ± 0.08 g) were allocated into five groups and fed either the basal diet (CPH 0) or the basal diet that replaced fish meal with 1% (CPH 1), 3% (CPH 3), 5% (CPH 5), and 7% CPH (CPH 7). Dietary CPH 3 increased the activities of protease, Na⁺/K⁺‐ATPase, amylase, creatine kinase, and γ‐glutamyl transpeptidase; microvillus length in the anterior and mid intestines; and the mRNA levels of GH, growth hormone receptor (GHR), and IGF‐I without any negative effects on growth. Dietary CPH 7 increased the mRNA levels of tumor necrosis factor‐α, interleukin‐6, IκB kinase alpha, and nuclear factor κB but decreased the transcript level of inhibitor of κB‐α (p < .05). Therefore, replacing fish meal with CPH 3 improved intestinal function and the transcription of GH/IGF‐I axis genes without any adverse effect on growth or triggering of inflammation in juvenile blunt snout bream.

中文翻译：

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用棉籽粉蛋白水解物代替鱼粉会影响少年钝嘴鲷（Megalobrama amblycephala）的生长，肠功能和生长激素/胰岛素样生长因子I轴

这项研究旨在评估用棉籽粉蛋白水解物（CPH）代替鱼粉对生长，肠功能，生长激素/胰岛素样生长因子I（GH / IGF‐I）轴和钝嘴鲷的炎症的影响，巨型lo脑。总共将300条鱼（38.66±0.08 g）分为五组，分别喂食基础饲料（CPH 0）或用1％（CPH 1），3％（CPH 3），5代替鱼粉的基础饲料％（CPH 5）和7％CPH（CPH 7）。日粮CPH 3可增加蛋白酶，Na ⁺ / K ⁺ -ATPase，淀粉酶，肌酸激酶和γ-谷氨酰转肽酶的活性。前肠和中肠的微绒毛长度；GH和生长激素受体（GHR）的mRNA水平;）和IGF-I对生长没有任何负面影响。日粮CPH 7增加了肿瘤坏死因子α，白细胞介素6，IκB激酶α和核因子κB的mRNA水平，但降低了κB‐α抑制剂的转录水平（p  <.05）。因此，用CPH 3代替鱼粉可改善肠道功能，并改善GH / IGF-I轴基因的转录，而对少年钝嘴鼻鱼的生长或引发炎症没有任何不利影响。