As a result of the detection of the pinewood nematode Bursaphelenchus xylophilus in Portugal, and its subsequent spread to Spain, intense surveys were conducted to screen for the presence of Bursaphelenchus species in Romania. Herein, we report recent surveys of insects potentially vectoring Bursaphelenchus species collected using trap trees or pheromone‐baited traps placed in the forest. Trap felled spruce trees (Picea abies) and pheromone‐baited traps were installed in six different counties in Romania (Brașov, Sibiu, Suceava, Hunedoara, Timiș and Dâmbovița). Ten different species of insects distributed among Curculionidae and Cerambycidae were obtained. Nematodes were extracted from insects and observed to validate the presence of Bursaphelenchus specimens. One female identified as Monochamus sutor was the only specimen carrying nematodes in the genus Bursaphelenchus. Nematodes were identified as B. mucronatus based on morphological and molecular features. This is the first detection and report of natural spread of B. mucronatus in Romania. The absence of B. xylophilus was confirmed in the areas of Romania surveyed in this work.

中文翻译：

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罗马尼亚首次在 Monochamus sutor（鞘翅目：天牛科）上发现 Bursaphelenchus mucronatus（线虫科：Aphelenchoididae）

由于在葡萄牙检测到松材线虫 Bursaphelenchus xylophilus 并随后传播到西班牙，因此进行了密集调查以筛选罗马尼亚是否存在 Bursaphelenchus 物种。在此，我们报告了最近对使用陷阱树或放置在森林中的信息素诱饵陷阱收集的可能传播 Bursaphelenchus 物种的昆虫的调查。在罗马尼亚的六个不同县（布拉索夫、锡比乌、苏恰瓦、胡内多阿拉、蒂米什和丹博维萨）安装了捕集云杉树（Picea abies）和诱捕信息素的捕集器。获得了分布在仙茅科和天牛科的十种不同的昆虫。从昆虫中提取线虫并观察以验证 Bursaphelenchus 标本的存在。一名被确定为 Monochamus sutor 的雌性是唯一携带 Bursaphelenchus 属线虫的标本。根据形态和分子特征，线虫被鉴定为 B. mucronatus。这是罗马尼亚首次发现并报告了 B. mucronatus 的自然传播。在这项工作中调查的罗马尼亚地区中没有松材线虫被证实。