Wilt disease caused by Fusarium solani is a serious constraint to Dalbergia sissoo (shisham) plantations in northern India. In this study, the antagonistic potential of 40 bacterial isolates recovered from rhizophere soil of healthy shisham trees, and a well‐characterized Trichoderma species (Trichoderma virens) were tested for their possibility as biocontrol agents for F. solani. Two promising isolates (S1 and S15) were identified which inhibited pathogen growth, caused chitin degradation, produced siderophores and solubilized phosphate in vitro. Isolate S15 scored highest for hydrogen cyanide (HCN) production while isolate S1 was a non‐HCN producer. These two isolates were identified as Serratia marcescens (S1) and Pseudomonas azotoformans (S15) following sequence analysis of 16S rDNA. In dual culture assays, T. virens caused 80% inhibition of mycelial growth of the test fungus. The three selected antagonists when tested in planta in the glasshouse completely suppressed production of wilt symptoms on 12‐month‐old shisham plants. Further work is needed to ascertain the potential of these isolates to be used as biocontrol agents to manage shisham wilt under field conditions.

中文翻译：

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偶氮假单胞菌，粘质沙雷氏菌和维氏木霉对黄檀枯萎病的生物防治潜力。

由枯萎镰刀菌引起的枯萎病严重限制了印度北部的黄檀sissoo（shisham）人工林。在这项研究中，测试了从健康水草树的根茎土壤中回收的40种细菌分离物的拮抗潜力，以及一种特性良好的木霉菌种（Trichoderma virens）的抗微生物潜力，该菌株可用作茄形镰刀菌的生物防治剂。鉴定出两个有希望的分离物（S1和S15），它们在体外抑制病原体生长，引起甲壳质降解，产生铁载体和溶解的磷酸盐。菌株S15在氰化氢（HCN）生产中得分最高，而菌株S1是非HCN生产者。这两个分离株被鉴定为在对16S rDNA进行序列分析后，粘质沙雷氏菌（S1）和偶氮假单胞菌（Sseudomonas azotoformans）（S15）。在双重培养分析中，维氏梭菌引起测试真菌的菌丝体生长80％的抑制。当测试的三种选择的拮抗剂在植物在温室中完全抑制生产的萎蔫症状上12个月大的shisham植物。需要做进一步的工作来确定这些分离物在野外条件下作为生防shi的潜力。