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Identification of a phytoplasma associated with Syringa reticulata witches' broom disease in China
Forest Pathology ( IF 1.3 ) Pub Date : 2020-04-03 , DOI: 10.1111/efp.12592 Jing Yang 1 , Ya Jun Liao 2 , Jun Hui Ning 1 , Jin Zhong Wang 1 , He Wang 3 , Zheng Guang Ren 1
Forest Pathology ( IF 1.3 ) Pub Date : 2020-04-03 , DOI: 10.1111/efp.12592 Jing Yang 1 , Ya Jun Liao 2 , Jun Hui Ning 1 , Jin Zhong Wang 1 , He Wang 3 , Zheng Guang Ren 1
Affiliation
During the summer of 2018, one Syringa reticulata plant showing witches' broom and small leaves was observed in Beijing, China. Molecular diagnostic tools and electron microscopic cell observation were used to detect the possible pathogen of this disease. As a result, the phytoplasma in the symptomatic S. reticulata tree was confirmed by amplifying the 16S rRNA gene using the phytoplasma‐specific universal primer pair R16mF2/R16mR1 and observation with transmission electron microscopy. The rp and tuf genes of the phytoplasma were also cloned and sequenced as the 16S rRNA gene. Sequence and phylogenetic analyses of the 16S rRNA, rp and tuf genes indicated that the phytoplasma associated with S. reticulata witches' broom (SrWB) disease belonged to the 16SrV‐B subgroup, and it was closely related to the 16SrV‐B subgroup phytoplasma strain jujube witches' broom, which causes serious disease of jujube fruit trees in China. This study shows the S. reticulata tree as a new host of a phytoplasma belonging to the 16SrV‐B subgroup in China.
更新日期：2020-04-03
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