Raffaelea quercus‐mongolicae is a mycangial symbiont of a wood‐boring ambrosia beetle, Platypus koryoensis (Coleoptera: Curculionidae: Platypodidae), which is believed to be associated with oak mortality in South Korea. Since the first discovery of the disease in 2004, oak mortality has continued to spread across the country, and the pathogen has had a significant impact on forest ecosystems in the country; however, little is known regarding the biology of the fungus. The aim of this study was to develop markers to determine the mating types of individual isolates of R. quercus‐mongolicae collected across the country and then determine the mating‐type ratio in natural populations. To achieve this, partial sequences of MAT1‐1–3 and MAT1‐2–1 genes were amplified using degenerate primers and cloned. The new primer sets, which were specific to each mating‐type idiomorph, identified isolates as MAT1‐1 and MAT1‐2 mating types. The roughly equal frequencies of the two mating types suggest that sexual reproduction might have occurred in natural populations of R. quercus‐mongolicae in South Korea.

中文翻译：

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韩国红aff栎的交配型分布调查

桔梗（Raffaelea quercus-mongolicae）是一种木材枯燥的甲虫Platypus koryoensis（鞘翅目：Curculionidae：Platypodidae）的真菌共生体，据信与韩国的橡树死亡有关。自从2004年首次发现该病以来，橡树的死亡率一直在全国范围内蔓延，病原体对该国的森林生态系统产生了重大影响。然而，关于真菌的生物学知之甚少。这项研究的目的是开发标记物，以确定在全国范围内采集的蒙古栎的分离菌株的交配类型，然后确定自然种群中的交配类型比率。为了达到这个目的，使用简并引物扩增MAT1–1–3和MAT1–2–1基因并进行克隆。针对每种交配型独特型的新引物组将分离株鉴定为MAT1-1和MAT1-2交配类型。两种交配类型的频率大致相等，这表明自然繁殖的R种群可能发生过性繁殖。 韩国的蒙古栎。