Nowadays, petroleum pollution is one of the most important environmental challenges in Iran. Some bacteria were isolated for hexadecane degrading from soil, sediment, and sludge; however, there is no report on its isolation from compost. This study was aimed to isolation, molecular identification of novel bacteria with high capability of hexadecane-degrading from compost using enrichments media. The isolated bacteria were identified by PCR with 16S rDNA method and were studied their ability for removing hexadecane in liquid and solid medium. According to results, the isolated bacteria were identified as O.oryzae and P.lautus. In liquid medium, hexadecane concentration decreased from 3000±1.4 to 366.96±0.9 mg/l (87.77±0.2%) by O.oryzae, while the removal percent by P.lautus was 80.89±1.2% after 33 days. Hexadecane concentration decreased from 30 to 18.09±0.6 g/kg soil (39.69±1.1%) during 80 days. The pseudo-first order was the best model for biodegradation by both cultures. Whereas, the kinetic behavior of hexadecane bioremediation was described by first and pseudo-first-order model (r² = 0.798). In addition, these isolated bacteria have higher efficiency in hexadecane removal in comparison to the other previously identified bacteria. Moreover, O.oryzae can be used in remediation of petroleum products, especially diesel oil.

如今，石油污染是伊朗最重要的环境挑战之一。从土壤，沉积物和污泥中分离出一些细菌进行十六烷降解。但是，没有关于将其与堆肥隔离的报道。这项研究旨在使用富集培养基从堆肥中分离出具有十六烷高降解能力的新型细菌，并对其进行分子鉴定。通过PCR和16S rDNA方法鉴定分离的细菌，并研究其在液体和固体培养基中去除十六烷的能力。根据结果​​，分离出的细菌被鉴定为O.oryzae和P.lautus。在液体培养基中，十六烷浓度为3000±1.4通过降低至366.96±0.9毫克/升（87.77±0.2％）O.oryzae，而33天后月桂对虾的去除率为80.89±1.2％。在80天内，十六烷浓度从30降低至18.09±0.6 g / kg土壤（39.69±1.1％）。假一阶是两种培养物进行生物降解的最佳模型。而十六烷生物修复的动力学行为由一阶和拟一阶模型描述（r ² = 0.798）。另外，与其他先前鉴定的细菌相比，这些分离的细菌在十六烷去除方面具有更高的效率。此外，米曲霉可用于修复石油产品，尤其是柴油。