Development of specific microsatellite primers is a relatively expensive and time-consuming process, so transferring already developed markers from one species to others is desirable, especially for non-model organisms with large genome structure such as conifers. In austral South America, the three Cupressaceae are monotypic genera of high conservation and evolutionary relevance. Although they had been the focus of numerous population genetic studies the lack of highly polymorphic markers as microsatellites for all species, has limited the possibility of evolutionary studies and the design of appropriate conservation plans. Thus the aim of this study is to transfer and test microsatellites primers early designed for Austrocedrus to Fitzroya and Pilgerodendron. Transferability of nine microsatellite primers and their utility were tested by comparing genetic parameters of 20 individuals of each species in six populations where the three species occur. Four markers were simultaneously transferred with success, representing the first study in conifers to achieve cross-genera transferability. This small set of primers yielded high levels of polymorphism for the three studied species and show unique alleles that separate pure taxa. These highly polymorphic markers provide relevant tools to study the evolutionary history of South American Cupressaceae and to generate information that could help to guide conservation efforts.

特定微卫星引物的开发是一个相对昂贵且耗时的过程，因此需要将已开发的标记物从一个物种转移到另一个物种，特别是对于具有较大基因组结构的非模式生物，例如针叶树。在南美南方，三个柏科是具有高度保护和进化相关性的单型属。尽管它们一直是众多种群遗传学研究的重点，但由于缺乏用于所有物种的微卫星的高度多态性标记，因此限制了进行进化研究和设计适当的保护计划的可能性。因此，本研究的目的是将早期为奥氏体设计的微卫星引物转移和测试至Fitzroya和Pilgerodendron。通过比较三个物种出现的六个种群中每个物种的20个个体的遗传参数，测试了九种微卫星引物的可转移性及其实用性。四个标记同时成功转移，这是针叶树中实现跨属转移性的第一个研究。这种少量的引物为三个研究物种产生了高水平的多态性，并显示了分离纯生物群的独特等位基因。这些高度多态的标记物为研究南美柏科的进化史和产生有助于指导保护工作的信息提供了相关工具。