Abstract The aim was to determine the absence of gluten in pet food samples marked as ‘grain-free’ and ‘gluten-free’ diets, to assess the reliability of manufacturer labelling of such products. A total of 15 diets labelled as grain- or gluten-free and 2 commercial diets containing wheat were sampled. An analytical procedure using high-pressure liquid chromatography coupled with mass spectrometry with high power of resolution was developed and applied to determine specific markers of wheat gluten. The results are expressed as mg of wheat flour type ‘00’ present in 1 g of feed. The quantification limit (LOQ) obtained in the flour for ion m/z 894.5043, z = 2, is 4 mg of flour per gram. In 14 out of the 15 samples from a grain- or gluten-free diet the quantifier ion signal was < LOQ, while in 1 out of the 15 samples 10 mg of flour/g feed were measured. Highlights Adverse reaction to gluten in dogs have been documented in certain breeds Gluten is tricky to detect and measure in pet food Contamination of gluten in pet food samples marked as ‘grain-free’ and ‘gluten-free’ diets An analytical procedure was developed using HPLC coupled with HRMS

中文翻译：

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HPLC-HRMS 测定罐装和干燥无谷物商业宠物食品的麸质污染

摘要 目的是确定标记为“无谷物”和“无麸质”饮食的宠物食品样品中不含麸质，以评估此类产品的制造商标签的可靠性。总共抽取了 15 种标记为无谷物或无麸质的饮食和 2 种含有小麦的商业饮食。开发了一种使用高压液相色谱与具有高分辨能力的质谱联用的分析方法，并将其应用于确定小麦面筋的特定标记物。结果表示为 1 克饲料中存在的“00”型小麦粉的毫克数。在面粉中获得的离子 m/z 894.5043 (z = 2) 的定量限 (LOQ) 是每克面粉 4 mg。在来自无谷物或无麸质饮食的 15 个样品中，有 14 个样品的定量离子信号 < LOQ，而在 15 个样品中，有 1 个样品的面粉含量为 10 毫克/克饲料。亮点 某些品种的狗对麸质有不良反应 宠物食品中的麸质难以检测和测量 标记为“无谷物”和“无麸质”饮食的宠物食品样品中的麸质污染 我们开发了一种分析程序HPLC 结合 HRMS