ABSTRACT Tetranychus urticae Koch is a common pest of many crops in Turkey and the world. In this study, abamectin LC values, resistance rates, detoxification enzyme activities and the presence of G314D point mutation in the target site were investigated in T. urticae collected from cut flower production greenhouses in Antalya. The LC values of the susceptible and field populations were determined by spray tower-leaf disc method. The resistance rates of field populations ranged from 1.60 to 850.12-fold compared to the susceptible population. Activities of three detoxification enzymes were determined by a microplate reader. The activity of esterase and GST ranged from 4.04 to 8.21 and from 4.40 to 11.69 mOD min−1 mg−1 proteins, respectively, in different populations. On the other hand, the P450 enzyme activity of the field populations was 0.52-1.80 RFU 30 min-1 mg-1 protein. Screening the populations for the presence of G314D point mutation in glutamate-gated chloride channels (GluCls) by real-time PCR using the mutation-specific TaqMan probe revealed no mutations in any populations tested. The results showed that the resistance in the populations tested in this study is likely associated with activity of detoxifying enzymes rather than target site mutations in the GluCls channels.

中文翻译：

---

土耳其切花温室叶螨种群对阿维菌素的抗性及抗性机制

摘要 Tetranychus urticae Koch 是土耳其和世界上许多作物的常见害虫。在这项研究中，从安塔利亚切花生产温室收集的荨麻中，研究了阿维菌素 LC 值、抗性率、解毒酶活性和目标位点 G314D 点突变的存在。感病种群和田间种群的LC值采用喷雾塔叶盘法测定。田间种群的耐药率是易感种群的 1.60 至 850.12 倍。三种解毒酶的活性由酶标仪测定。在不同人群中，酯酶和 GST 的活性范围分别为 4.04 至 8.21 和 4.40 至 11.69 mOD min-1 mg-1 蛋白质。另一方面，田间种群的P450酶活性为0.52-1。80 RFU 30 min-1 mg-1 蛋白质。使用突变特异性 TaqMan 探针通过实时 PCR 筛选群体中是否存在 G314D 点突变的谷氨酸门控氯通道 (GluCls)，结果显示在任何测试群体中均未发现突变。结果表明，本研究中测试的人群中的耐药性可能与解毒酶的活性有关，而不是与 GluCls 通道中的靶位点突变有关。