ABSTRACT Male flower induction and androgenesis in three genotypes of cucumber (Cucumis sativus), two Beth alpha F1 hybrids (BT1 and BT2, gynoecious) and Dastgerdi (DTG, monoecious), were evaluated. Induction of male flowers in gynoecious cucumber cultivars was assessed by single and combinatorial spraying of AgNO3 (3 mM), GA3 (1.5 mM) and CoCl2 (3 mM) after the first true leaf emergence. After 6 weeks from the beginning of treatments, the average numbers of male flowers induced by spraying AgNO3, GA3, and CoCl2 were 42, 14.5, and 0, respectively. Androgenesis was performed in two gynoecious cultivars, in which the donor plants were treated with AgNO3 (3 mM) or GA3 (1.5 mM) and the DTG cultivar without treatment. Among the three cultivars, the DTG showed the highest percentage of embryogenic calli formation (62.2%) and number of embryos per anther (1.81). In the gynoecious cultivars, the percentage of embryogenic calli formation (27.1%) and number of embryos per anther (0.23) in the anthers of GA3 treated plants were significantly higher than those treated by AgNO3. Cytological and SSR marker analysis of regenerants showed that 16 plants were spontaneous doubled haploid, 2 plants were diploid and one plant was tetraploid.

中文翻译：

---

雄花诱导显着影响黄瓜 (Cucumis sativus L.) 的雄激素生成

摘要 评估了三种基因型黄瓜 (Cucumis sativus)、两种 Beth alpha F1 杂种（BT1 和 BT2，雌雄同株）和 Dastgerdi（DTG，雌雄同株）的雄花诱导和雄性生成。在第一次真叶出现后，通过单一和组合喷洒 AgNO3 (3 mM)、GA3 (1.5 mM) 和 CoCl2 (3 mM) 来评估雌性黄瓜栽培品种对雄花的诱导。处理开始6周后，喷施AgNO3、GA3和CoCl2诱导的雄花平均数分别为42、14.5和0。雄激素在两个雌性栽培品种中进行，其中供体植物用 AgNO3 (3 mM) 或 GA3 (1.5 mM) 处理，而 DTG 栽培品种未经处理。在这三个品种中，DTG 显示出最高的胚性愈伤组织形成百分比 (62. 2%) 和每个花药的胚胎数 (1.81)。在雌性品种中，GA3处理的植物花药中胚性愈伤组织形成百分比（27.1%）和每花药胚数（0.23）均显着高于AgNO3处理。再生子的细胞学和SSR标记分析表明16株植物为自发双单倍体，2株为二倍体，1株为四倍体。